The nuclear change of tobacco transformed with chromatin of Chlamydomonas reinhardtii and the expression of nuclear gene

doi:10.7525/j.issn.1673-5102.2005.04.012

Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (4): 432-435.doi: 10.7525/j.issn.1673-5102.2005.04.012

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The nuclear change of tobacco transformed with chromatin of Chlamydomonas reinhardtii and the expression of nuclear gene

ZU Yuan-Gang, GUO Xiao-Rui, WU Shuang-Xiu, MENG Qing-Huan, LI Yan-Hua, ZHANG Zhong-Hua, ZHANG Yu-Liang, SUN Jia-Yin

Key Laboratory of Forestry Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040

Received:2005-07-17 Online:2005-12-15 Published:2016-06-14
Supported by:
Supported by the State Key Basic Research Project (G19990160) and the Key Project of Chinese Ministry of Education (104191)

Abstract

Abstract: Through the method of being frozen in the liquid nitrogen for a short time, and then thawing in the room temperature for several times, the protoplasts of Chlamydomonas reinhardtii were broken and chromatin were obtained.Then we exactly transformed the chromatin to the explants of the tobacco leaves by the micromanipulation, and then we cultured the explants, observed by the fluorescent microscope every day.The result indicated that the chromatin transformed explants, the nuclei of C. reinhardtii and tobacco were all changed.At the same time, the explants formed the callus and then grew into gemmule.Through the RT-PCR detection, we found the expression of the nuclear gene rbcs2.

Key words: Chlamydomonas reinhardtii, chromatin, micromanipulation, RT-PCR

ZU Yuan-Gang, GUO Xiao-Rui, WU Shuang-Xiu, MENG Qing-Huan, LI Yan-Hua, ZHANG Zhong-Hua, ZHANG Yu-Liang, SUN Jia-Yin. The nuclear change of tobacco transformed with chromatin of Chlamydomonas reinhardtii and the expression of nuclear gene[J]. Bulletin of Botanical Research, 2005, 25(4): 432-435.

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The nuclear change of tobacco transformed with chromatin of Chlamydomonas reinhardtii and the expression of nuclear gene

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