Abstract: Tea is a healthy food, beacause it has various bioactive components. Recent research indicated that tea glycoprotein might be a bioactive component in tea. In this report, total proteins were extracted from tea fresh leaves, and coarse glycoproteins were isolated through the methods of ammonium sulfate fractions (45%~70%) and SephadexG-100 chromatography. According to control staining of glycan and polypeptide, glycoproteins were determinated by using Pierce GelCode© Glycoprotein Staining Kit and Coomassie Brilliant Blue R250, and three tea glycoproteins were fast purified from the SDS-gels. Sixteen native bioactive glycoproteins were gained from tea total proteins by ConA-Sepharose 4B affinity chromatography.

Key words: tea plant, glycoprotein, isolation, determination, ConA-Sepharose 4B affinity chromatography