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植物研究 ›› 2007, Vol. 27 ›› Issue (2): 182-185.doi: 10.7525/j.issn.1673-5102.2007.02.014

• 论文 • 上一篇    下一篇

春萝卜抽薹过程中内源激素含量变化分析

宋贤勇1,2;柳李旺1*;龚义勤1;黄丹琼1;汪隆植1   

  1. 1.南京农业大学作物遗传与种质创新国家重点实验室,园艺学院,南京 210095 2.江苏中江种业股份有限公司,南京 210009
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-03-20 发布日期:2007-03-20
  • 通讯作者: 柳李旺
  • 基金资助:
     

Hormone Content and Balance During Bolting of Spring Radish (Raphanus sativus L.)

SONG Xian-Yong;LIU Li-Wang*;GONG Yi-Qin;HUANG Dan-Qiong;WANG Long-Zhi   

  1. 1.National Key Laboratory of Crop Genetics and Germplasm Enhancement; College of Horticulture, Nanjing Agricultural University,Nanjing 210095 2.Jiangsu Zhongjiang Seed Co.,Ltd.,Nanjing 210009
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-03-20 Published:2007-03-20
  • Contact: LIU Li-Wang
  • Supported by:
     

摘要: 采用酶联免疫吸附法(ELISA)测定了春萝卜抽薹过程中顶端生长点处嫩叶的赤霉素(GA4)、生长素(IAA)、细胞分裂素(iPA)和脱落酸(ABA)等含量的变化情况。研究结果表明,在萝卜抽薹过程中,GA4含量在现蕾点存在明显的峰值,表明GA4与现蕾抽薹密切相关,起着主导作用;内源ABA含量变化与GA4变化趋势基本一致;与GA4和ABA含量相比,抽薹过程中叶片IAA含量相对较低;iPA含量在现蕾期逐步增加,至抽薹中后期出现一高峰值。研究发现iPA/ABA值增大,GA4/iPA和GA4/IAA值变小有利于花芽分化后萝卜植株抽薹开花,表明内源激素之间的平衡对萝卜抽薹开花也起着重要作用。

关键词: 萝卜, ELISA, 内源激素, 抽薹

Abstract: Endogenous hormones, including gibberellin (GA4), indole acetic acid (IAA), isopentenyl adenine (iPA) and abscisic acid (ABA), were analyzed during bolting in spring radish (Raphanus sativus L.). Tissue from young leaves near the apex was analyzed with the ELISA. The results showed that an obvious peak value of GA4 content occurred at the bud-emerging point, indicating that this hormone is an important contributor to the bolting process. The ABA had a similar trend to that of GA4, while the IAA content was relatively low compared with that of GA4 and ABA. The content of iPA increased gradually during the bud-emerging stage and reached a maximum value at the late bolting period. The results also demonstrated that the balance of these endogenous hormones play an important role in the bolting process. A high ratio of iPA/ABA and low ratio of GA4/iPA and GA4/IAA were beneficial to floral bud differentiation and bolting in radish.

Key words: radish, ELISA, endogenous hormone, bolting

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