欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2010, Vol. 30 ›› Issue (1): 106-110.doi: 10.7525/j.issn.1673-5102.2010.01.019

• 论文 • 上一篇    下一篇

珍稀植物跳舞草的组织培养与快速繁殖

毛艳萍1,2;苏智先2*;邹利娟2;吴庆贵2   

  1. 1.西华师范大学生命科学学院,南充 637002;2.四川省生态与环境技术重点实验室,绵阳 621000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-20 发布日期:2010-01-20
  • 通讯作者: 苏智先
  • 基金资助:
     

Tissue Culture and Rapid Propagation of Rare Plant in Codariocalyx motorius(Houtt.)

MAO Yan-Ping;SU Zhi-Xian*;ZOU Li-Juan;WU Qing-Gui   

  1. 1.College of Life Science,China West Normal University,Nanchong 637002;2.Sichuan Provincial Key Laboratory of Ecology and Environmental Technology,Mianyang 621000
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
  • Contact: SU Zhi-Xian
  • Supported by:
     

摘要: 以跳舞草无菌苗为实验材料,以MS、1/2MS为基本培养基,研究不同条件对跳舞草快速繁殖的影响,初步建立了跳舞草组培快繁体系,筛选出最佳愈伤组织诱导及不定芽分化培养基为MS+6-BA 2.0 mg·L-1+NAA 0.1 mg·L-1+蔗糖30.0 g·L-1+VC 2.0 mg·L-1,最佳增殖培养基为MS+6-BA 2.0 mg·L-1+NAA 0.05 mg·L-1,最佳生根培养基为1/2MS+NAA 0.2 mg·L-1+IAA 0.5 mg·L-1

关键词: 跳舞草, 不定芽增殖, 组织培养, 快速繁殖

Abstract: The aseptic seedings of Codariocalyx motorius(Houtt.) Ohashi were the materials of our study on issue culture and rapid propagation, MS and 1/2MS were the basic mediums. Impact of different conditions on the rapid propagation of C.motorius(Houtt.) was discussed and a tissue culture system of C.motorius(Houtt.) was initially established, the best callus adventitious and bud differentiation medium was MS+6-BA 2.0 mg·L-1+NAA 0.1 mg·L-1+sugar 30 g·L-1+VC 2.0 mg·L-1, the best medium for the shoot regeneration was MS+6-BA 2.0 mg·L-1+NAA 0.05 mg·L-1, the best rooting medium was 1/2MS+NAA 0.2 mg·L-1+IAA 0.5 mg·L-1.

Key words: Codariocalyx motorius(Houtt.), multiplication of adventitious buds, tissue culture, rapid propagation

中图分类号: