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植物研究 ›› 2008, Vol. 28 ›› Issue (1): 67-72.doi: 10.7525/j.issn.1673-5102.2008.01.015

• 论文 • 上一篇    下一篇

飞机草花蕾中Beclin1基因的克隆与表达

祁长青;刘英;王俐;李思滨;王敏;姜洋;祖元刚*   

  1. 东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-01-20 发布日期:2008-01-20
  • 通讯作者: 祖元刚
  • 基金资助:
     

Cloning and Characterization of Beclin1-ike Gene in Eupatorium odoratum L. buds

QI Chang-Qing;LIU Ying;WANG Li;LI Si-Bin;WANG Min;JANG Yang;ZU Yuan-Gang*   

  1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-20 Published:2008-01-20
  • Contact: ZU Yuan-Gang
  • Supported by:
     

摘要: 本文以入侵植物飞机草的不同时期花蕾为材料,利用RT-PCR法扩增得到了与PCD相关的类Beclin1基因的部分cDNA序列(大约700bp),与烟草叶片中的基因序列(AY701316)同源性为95%;Northern blotting结果表明类Beclin1基因在花蕾发育中期的表达量高于初期和后期;通过DNA ladder检测表明在花蕾发育过程中伴随着PCD的发生,这些结果表明花蕾发育中期是PCD初期发生的活跃期,是绒毡层逐渐退化和花粉不断形成的过程。通过对生殖过程中的细胞程序性死亡的分子生物学研究,初步揭示了飞机草入侵过程与PCD的相互关系。

关键词: 飞机草, 花蕾, Beclin1基因, 细胞程序化死亡, 绒毡层

Abstract: Beclin1-like gene was cloned from three different growth periods flower buds of Eupatorium odoratum by RT-PCR method in this study. The homology between Beclin1-like gene (about 700bp) and Beclin1 gene in tobacco leaves is 95%; Northern blotting result showed that the expression of Beclin1-like gene in the middle stage was higher than those in initial stages and dernier stage; the result of DNA ladder showed Programmed Cell Death (PCD) happened in the process of flower buds. All the results proved that PCD certainly happened with the tapetum degenerating and the pollen coming into being, which indicated the relationship between the aggressive process of E. odoratum and PCD in the process of the reproduction.

Key words: Eupatorium odoratum, buds, Beclin1 gene, Programmed Cell Death (PCD), tapetum

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