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植物研究 ›› 2005, Vol. 25 ›› Issue (2): 210-212.doi: 10.7525/j.issn.1673-5102.2005.02.021

• 论文 • 上一篇    下一篇

超声提取-高效液相色谱法测定甘草中甘草酸含量

付玉杰, 赵文灏, 侯春莲, 刘晓娜, 施晓光, 祖元刚   

  1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040
  • 收稿日期:2005-01-07 出版日期:2005-06-15 发布日期:2016-06-14
  • 通讯作者: 祖元刚 E-mail:zygorl@public.hr.h.lcn
  • 作者简介:付玉杰(1967-),女,博士,副教授,从事植物药基础研究与新药开发工作。
  • 基金资助:
    哈尔滨市科技攻关计划项目(2004AA3CS172-2)和东北林业大学科研启动基金

Determination of the Glycyrrhizic acid content from Glycyrrhiza uralensis by HPLC

FU Yu-Jie, ZHAO Wen-Hao, HOU Chun-Lian, LIU Xiao-Na, SHI Xiao-Guang, ZU Yuan-Gang   

  1. Key Laboratory of Forest Plant Ecolory, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2005-01-07 Online:2005-06-15 Published:2016-06-14

摘要: 确定了制备甘草酸分析样品的超声波提取条件, 0.3%稀氨水为溶剂,液固比50:1,提取时间5 h;确定了高效液相色谱法检测甘草酸含量的条件为ODS色谱柱(4.6mm×25 cm, 5 μm),检测波长254 nm,检测温度为室温,流动相CH3OH/3% HOA c(V/V)为75/25,流速1mL/m in,进样量10 μL,平均加样回收率100.20%,相对标准偏差为1.77%。结果表明超声提取-高效液相色谱法是一种准确度高,速度快的测定甘草中甘草酸含量的检测方法。

关键词: 甘草, 甘草酸含量, 超声波提取法, 高效液相色谱法(HPLC)

Abstract: Extracted Glycyrrhizic acid (GA) from Glycyrrhiza uralensis Fisch by Ultrasonic extraction method, the conditions with higher GA were 0.3% ammonia solution as solvent, ratio of liquid to solid as 50:1(mL/g), extraction time five hours. The HPLC analysis conditions were as follows, chromatographic column ODS, detecting wavelength 254 nm, room temperature, mobile phase CH 3OH/3% HOAc(V/V) which ratio was 75/25, flowing rate 1 mL/min,injected sample volume 10 μL. The result of percent recovery is 100.20%, the relative standard deviation is 1.77%. Ultrasonic extraction-HPLC is a fast detecting GA method with higher accuracy from Glycyrrhiza uralensis.

Key words: Glycyrrhiza uralensis Fisch, glycyrrhizic acid content, ultrasonic extraction, high performance liquid chromagraphy