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植物研究 ›› 2005, Vol. 25 ›› Issue (3): 298-303.doi: 10.7525/j.issn.1673-5102.2005.03.013

• 论文 • 上一篇    下一篇

镉胁迫下三个萝卜栽培种蛋白质变化的双向电泳比较研究

范宝莉, 王振英, 彭永康   

  1. 天津师范大学生物系, 天津 300074
  • 收稿日期:2005-01-17 出版日期:2005-09-15 发布日期:2016-06-14
  • 通讯作者: 彭永康 E-mail:PYKcell@eyou.com
  • 作者简介:FANBao-Li(1976-),Female,Master,Assistant,Majorincellandmolecularbiology.

Protein changes induced by cadmium stress in three radish cultivar seedlings: a comparison using two-dimensional gel electrophoresis

FAN Bao-Li, WANG Zhen-Ying, PENG Yong-Kang   

  1. Department of Biology, Tianjin Normal University, Tianjin 300074
  • Received:2005-01-17 Online:2005-09-15 Published:2016-06-14
  • Supported by:
    Supported by foundation for university key science and technology by the ministry of education of China(02010)

摘要: 在0.5mmol·L-1 Cd2+处理时,小五缨(XWY)的发芽率下降至92%,并且随着Cd2+浓度的增加,发芽率逐渐降低,在1和5mmol·L-1 Cd2+浓度处理时,发芽率分别降至83%和67%。象牙白(XYB)则在5mmol·L-1 Cd2+浓度时,发芽率下降。卫青(WQ)在0.05mmol·L-1 Cd2+浓度时,发芽率已降至83%, 1和5mmol·L-1时则下降至58%。幼苗生长也明显受Cd2+处理影响,在0.05mmol·L-1 Cd2+处理时, 3种栽培萝卜的幼苗生长均受到明显影响,并且随Cd2+浓度的增加,生长受抑加重。从发芽率和幼苗生长两种实验结果看,卫青对Cd2+最为敏感。双向电泳结果表明, Cd2+处理后3种栽培萝卜幼苗中蛋白质组分有明显变化。小五缨中, 0.1 mmol·L-1Cd2+处理后,有5个蛋白质点消失, 15个新的蛋白质点被诱导产生。象牙白中, 2个蛋白质点消失, 1个蛋白质点含量减少, 13个新的蛋白质点被诱导产生。卫青中, 12个新的蛋白质点诱导产生,但没有发现蛋白质点消失现象。Cd2+处理后, 3种栽培萝卜中,蛋白质合成的变化与幼苗生长受抑存在明显相关性,这一实验结果对于探讨萝卜Cd2+害的生化机理是有重要意义的。

关键词: 发芽率, 双向电泳, 蛋白质斑点, 镉, 萝卜

Abstract: The germination rate was decreased to 92% when seeds were treated at 0.5 mmol·L-1 Cd2+ and was progressively decreased with higher Cd2+. 83% and 67% germination rates were observed respectively at 1 mmol·L-1 and 5 mmol·L-1 Cd2+ treatment in XWY, but the lower germination rate was observed with 5 mmol·L-1 Cd2+ treatment in XYB. The germination rate was decreased to 83% only at 0.05 mmol·L-1 Cd2+ and only 58% germination rate was observed when seeds were treated at 1 mmol·L-1 and 5 mmol·L-1 Cd2+ in WQ. The effects of Cd2+ on seedling growth were observed in three radish cultivars. Seedling growth damage was much more serious with higher Cd2+ concentration. WQ is the most sensitive to Cd2+ treatment according to the experiment results which were obtained by testing germination rate and seedling growth in three radish cultivars. 5 protein spots were disappeared and 15 new protein spots were induced in XWY seedling which were treated by 0.1 mmol·L-1 Cd2+, 2 protein spots were disappeared and 1 protein spot was decreased in content, 13 new protein spots were induced in XYB, 12 new protein spots were induced in WQ. A close positive relationship was observed between protein spots change and inhibited seedling growth in the three radish cultivars which were treated by 0.1 mmol·L-1Cd2+. It may be important for understanding biochemical mechanism of Cd2+ damage.

Key words: germination rate, two dimensional gel electrophoresis, protein spot, cadmium, Raphanus sativus L.