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植物研究 ›› 2009, Vol. 29 ›› Issue (4): 509-512.doi: 10.7525/j.issn.1673-5102.2009.04.022

• 论文 • 上一篇    

灯盏花花药培养初报

张智慧;赵振玲;杨维泽;张金渝;杨美权;金航*   

  1. (云南省农业科学院药用植物研究所,昆明650231)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-07-20 发布日期:2009-07-20
  • 通讯作者: 金航
  • 基金资助:
     

Preliminary Study on Anther Culture of Erigeron breviscapus

ZHANG Zhi-Hui;ZHAO Zhen-Ling;YANG Wei-Ze;ZHANG Jin-Yu;YANG Mei-Quan;JIN Hang*   

  1. (Medicinal Plant Institute,Yunnan Academy of Agricultural Sciences,Kunming650231)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
  • Contact: JIN Hang
  • Supported by:
     

摘要: 对灯盏花花药培养诱导单倍体植株进行了研究。结果显示:灯盏花花药愈伤组织培养以附加60 g·L-1蔗糖较好,B5和MS培养基相比较,MS培养基较适宜,在MS+NAA 1.0 mg·L-1+BA 0.5 mg·L-1+蔗糖60 g·L-1的培养基中,花药愈伤组织诱导率可达36.03%。将愈伤组织转移到MS+6-BA 1.0 mg·L-1中继代增殖后,经芽苗分化、生根后可得到完整植株。再生植株根尖细胞经细胞学鉴定存在单倍体。

关键词: 灯盏花, 花药培养, 单倍体植株

Abstract: The preliminary study was carried out on the anther culture of Erigeron breviscapus. The results showed that during callus induction, 60 g·L-1 sucrose was suitable;MS medium was better than B5; the highest induction efficiency 36.03% was achieved on the medium of MS+NAA 1.0 mg·L-1+BA 0.5 mg·L-1+sucrose 60 g·L-1. calli were transferred on medium MS+6-BA 1.0 mg·L-1 to proliferation. After differentiating and taking roots, plantlets were obtained. The chromosome numbers from root-tip cells of regeneration plant was nine. It showed that the regeneration plant was haploid.

Key words: Erigeron breviscapus(Vant)Hand.-Mazz, anther culture, haploid

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