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植物研究 ›› 2020, Vol. 40 ›› Issue (1): 79-84.doi: 10.7525/j.issn.1673-5102.2020.01.012

• 研究报告 • 上一篇    下一篇

拟南芥根毛功能基因AtGDPD-Like3关键氨基酸位点鉴定

王爽, 程玉祥, 夏德安   

  1. 东北林业大学林木遗传育种国家重点实验室, 哈尔滨 150040
  • 收稿日期:2019-04-24 出版日期:2020-01-05 发布日期:2020-02-21
  • 通讯作者: 夏德安 E-mail:xiadean@126.com
  • 作者简介:王爽(1993-),女,硕士研究生,主要从事林木次生生长研究。
  • 基金资助:
    国家自然科学基金(31570580)

Identification of Key Amino Acid Sites in AtGDPD-Like3 with Role in Arabidopsis Root Hairs

WANG Shuang, CHENG Yu-Xiang, XIA De-An   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-04-24 Online:2020-01-05 Published:2020-02-21
  • Supported by:
    Natural Scientific Foundation of China(31570580)

摘要: AtGDPD-Like3是编码甘油磷酸二酯磷酸二酯酶(GDPD)类似基因,拟南芥该家族基因AtGDPD-Like3突变体shv3存在严重的根毛发育缺陷。为了鉴定AtGDPD-Like3关键氨基酸位点,我们构建S538A、V556A和D628A单点突变AtGDPD-Like3,分别转化atgdpdl3突变体并观察其恢复根毛缺陷程度。结果显示V556A、D628A位点突变AtGDPD-Like3完全恢复atgdpdl3根毛生长缺陷表型,但S538A突变AtGDPD-Like3只是部分恢复根毛缺陷。这些结果表明Ser538是AtGDPD-Like3较为关键的氨基酸位点,突变影响其蛋白质功能行使,同时暗示AtGDPD-Like3还存在其它的关键氨基酸位点。此研究结果为进一步探究AtGDPD-Like3蛋白功能行使的作用机制奠定了基础。

关键词: 拟南芥, AtGDPD-Like3, 关键氨基酸位点, 点突变

Abstract: AtGDPD-Like3 encode glycerophosphodiester phosphodiesterase-like(GDPD) proteins and shv3, its family gene AtGDPD-Like3 mutant, has serious root hair defects. To identify key amino acid sites in AtGDPD-Like3, we constructed S538A, V556A and D628A single site mutation of AtGDPD-Like3, which were expressed in atgdpdl3 mutants, respectively. The results showed that AtGDPD-Like3 with V556A or D628A mutation could completely restore root hairs growth defects of atgdpdl3, whereas, AtGDPD-Like3 with S538A mutation partially restored root hairs growth defects. These indicate that Ser538 is the key amino acid site in AtGDPD-Like3, which affects function of its protein. Meanwhile, it implies other key amino acid sites in AtGDPD-Like3. The results of this study would lay a foundation for further exploring the functional mechanism of AtGDPD-Like3 protein.

Key words: Arabidopsis thaliana, AtGDPD-Like3, key amino acid locus, point mutation

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