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植物研究 ›› 2006, Vol. 26 ›› Issue (2): 193-197.doi: 10.7525/j.issn.1673-5102.2006.02.015

• 论文 • 上一篇    下一篇

转抗真菌病基因烟草后代遗传分析

王占斌1;孙仲平2;张福丽1;张彦妮1;徐香玲3*   

  1. 1.东北林业大学林学院,哈尔滨 150040 2.哈尔滨师范大学附属中学,哈尔滨 150040 3.哈尔滨师范大学生物系,哈尔滨 150080
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-03-20 发布日期:2006-03-20
  • 通讯作者: 徐香玲
  • 基金资助:
     

Genetic analysis to descendants of transgene tobacco for control fungal disease

WANG Zhan-Bin;SUN Zhong-Ping;ZHANG Fu-Li;ZHANG Yan-Ni;XU Xiang-Ling*   

  1. 1. Northeast Forestry University,Harbin 150040 2. High school Atached Harbin Normal University,Harbin 150080 3. Harbin Normal University,Harbin 150080
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-03-20 Published:2006-03-20
  • Contact: XU Xiang-Ling
  • Supported by:
     

摘要: 利用载体pBLGC 通过发根农杆菌(Agrobacterium rhizogines)叶盘法转化烟草。对烟草转基因植株后代从DNA水平、RNA水平以及几丁质酶基因和β-1,3葡聚糖酶基因表达效率方面进行系统的研究。对探讨转基因植株后代遗传规律具有重要的意义。

关键词: 转基因, 后代, 遗传分析

Abstract: Vector of pBLGC by Agrobacterium rhizogines were used to transform tobacco, chitinase gene and β-1,3 glucanase gene were introduce into tobacco. Descendants of transgenic tobacco were detected from DNA level,RNA leval to expriment of control fungal disease, it is very important to research transgenic descendants heredity regular pattern.

Key words: transgene, descendants, genetic analysis

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