To clarify the differences of primary metabolism between Acanthopanax senticosus and A. sessiliflorus in different organs， and to explore their pharmacodynamic differences， medicinal value， and resource utilization structure， the metabolomics research strategy and GC-MS technology were used as methods. The results showed that 186 primary metabolites were identified in different parts of the two medicinal plants， and there were differences in primary metabolism between the two species. Further analysis showed that 52 differential metabolites in roots， 34 differential metabolites in stems， 39 differential metabolites in leaves， and 48 differential metabolites in petioles， were obtained respectively. The main metabolic pathways involved in differential metabolites were the aminoacyl tRNA biosynthesis pathway； biosynthetic pathways of valine， leucine， and isoleucine； glycine， serine， and threonine metabolic pathways； biosynthetic pathway of arginine； alanine， aspartate， and glutamate metabolic pathways； pyruvate metabolic pathway， respectively. In addition， there were differences in the levels of four key metabolites connecting primary metabolism and secondary metabolism in different organs respectively. A. senticosus and A. sessiliflorus were significantly different in the primary metabolism of different organs， which might be one of the reasons for the differences in pharmacological effects and secondary metabolism of different organs between the two species.
