To reveal the distribution of Arabinogalactan proteins（AGPs）of Ziziphus jujuba ‘Lingwu Changzao’during fruit development， fruits in the early bulking period， rapid enlargement period， coloring period and maturation period were used as materials and the distribution of AGPs were identified by methods of histochemistry and immunofluorescence localization respectively. The results showed the brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody distributed in the cell walls and cell interiors of the epicarp and the alongside internal layers of closely arranged mesocarp small cells in different periods fruit. The brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody distributed in the cell walls and the inner part of the large ovoid parenchyma cells in the inner mesocarp during the early bulking period， while they were mainly distributed in the parenchyma cell walls during the rapid enlargement period， coloring period and maturation periods， with no distribution in the inner part of most cells. With the ripening of fruits， the mesocarp parenchyma cells became more loosely arranged with elongated intercellular spaces， cell rupture occurred， and the distribution of brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody gradually decreased. The brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody distributed in the cell walls and cell interiors of all cells in the vascular bundle sheaths， xylem， phloem， and cambium of vascular bundles in different periods fruit respectively， and the number and size of vascular bundles gradually decreased with fruit development and further increased in size， and the distribution of brown red precipitate formed by βGlcY-AGPs and antigen recognized by MAC204 antibody gradually decreased. The results suggested that βGlcY and MAC204 were respectively effective method for histochemical localization and good antibody for immunofluorescence of Z. jujuba ‘Lingwu Changzao’ fruit， and there were some differences in brown red precipitate formed by βGlcY and the fluorescence intensity of antigen recognized by MAC204 antibody in different tissues of fruits at different periods， AGPs probably participated in morphogenesis during vascular bundles development of Z. jujuba ‘Lingwu Changzao’ fruit， which provided protection and nutritional support and polysaccharide accumulation for fruit development.
