Abstract: Woodsiaceae and Peranemaceae have always been considered to be two close relatives of families by many scholars, and their systematic positions have long been controversial, which have been placed in Aspidiaceae, Dryopteridoideae (Dennstaedtiaceae) and Athyrioideae (Dryopteridaceae). Nucleotide sequences of the chloroplast trnL-F region from 1 species of Woodsiacear,three species of Peranemaceae,eight species of Dryopteridaceae, two species of Dryopteriaceae and one species inoutgroup (Asplenium pseudolaserpitiifolium) were analyzed to gain insights into intergeneric relationships. The phylogenetic relationship was inferred using the neighbor-joining and maximum-parsimony methods, and both methods produced largely congruent trees. These trees reveal that: The relationship of Woodsiaceae and Peranemaceae is farther, Woodsiaceae is close to Athyriaceae, Peranemaceae is sister to Dryopteridaceae, Woodsiaceae and Peranemaceae are belong to different evolution lines. It is suitable that Ching (1978) and Pichi-Sermolii (1977) treeted them as independent families.

Key words: Woodsiaceae, Peranemaceae, trnL-F region, systematic position