Differences in Leaf Metabolism of Wild Rosa acicularis and Rosa acicularis ‘Luhe’ Based on GC-MS

doi:10.7525/j.issn.1673-5102.2022.06.017

Abstract

Abstract:

In order to explore the differences in the metabolites and metabolic pathways of leaves from wild Rosa acicularis and R. acicularis‘Luhe’，a GC-MS non-targeted metabolomics test was carried out on the leaves of two kinds of R. acicularis，6 amino acids，12 sugars，four alkanes，17 organic acids，five esters and seven other compounds and a total of 51 metabolites were detected respectively. The PCA model was in line with expectations and the OPLS-DA model screened out 19 significantly different metabolites. The analysis of differential metabolic pathways revealed a total of five major differential metabolic pathways. Based on the results， there were significant differences in the metabolism of some organic acids and sugars in as well as in the ABC transporter， phenylpropane biosynthesis， benzoic acid degradation and the metabolism of alanine， aspartic acid and glutamic acid in the leaves of the two species of R. acicularis. It was speculated that there were significant differences in leaf photosynthetic efficiency and resistance to stress， but further study was needed in combination with genome， proteome， transcriptome and other methods.

Key words: wild, Rosa acicularis, Rosa acicularis‘Luhe’, GC-MS metabolomics, differential metabolites, metabolic pathways

CLC Number:

S793.9

Yan WU, Sai LI, Kexin WU, Liqiang MU. Differences in Leaf Metabolism of Wild Rosa acicularis and Rosa acicularis ‘Luhe’ Based on GC-MS[J]. Bulletin of Botanical Research, 2022, 42(6): 1070-1078.

Figures/Tables 7

Table 1

Elements of metabolites of leaves from 2 R. acicularis

ID	名称 Name	分组 Class	ID	名称 Name	分组 Class
1	天冬氨酸Aspartic acid	氨基酸Amino acid	27	咖啡酸Caffeic acid	有机酸Organic acids
2	甘氨酸Glycine	氨基酸Amino acid	28	绿原酸Chlorogenic acid	有机酸Organic acids
3	L-丙氨酸L-Alanine	氨基酸Amino acid	29	柠檬酸Citric acid	有机酸Organic acids
4	L-脯氨酸L-Proline	氨基酸Amino acid	30	延胡索酸Fumaric acid	有机酸Organic acids
5	丝氨酸Serine	氨基酸Amino acid	31	没食子酸Gallic acid	有机酸Organic acids
6	缬氨酸Valine	氨基酸Amino acid	32	甘油酸Glyceric acid	有机酸Organic acids
7	β-龙胆二糖 beta-Gentiobiose	糖类Carbohydrate	33	乳酸Lactic Acid	有机酸Organic acids
8	阿拉伯呋喃糖Arabinofuranose	糖类Carbohydrate	34	苹果酸Malic acid	有机酸Organic acids
9	纤维二糖Cellobiose	糖类Carbohydrate	35	棕榈酸Palmitic Acid	有机酸Organic acids
10	D-果糖D-Fructose	糖类Carbohydrate	36	奎宁酸Quininic acid	有机酸Organic acids
11	葡萄糖Glucose	糖类Carbohydrate	37	莽草酸Shikimic acid	有机酸Organic acids
12	D-来苏糖D-Lyxose	糖类Carbohydrate	38	硬脂酸Stearic acid	有机酸Organic acids
13	D-甘露糖D-Mannose	糖类Carbohydrate	39	酒石酸Tartaric acid	有机酸Organic acids
14	L-鼠李糖L-Rhamnose	糖类Carbohydrate	40	1-棕榈酸单甘油酯1-Monopalmitin	酯类Mononitrate
15	L-苏氨酸L-Threonine	糖类Carbohydrate	41	硬脂酸甘油酯Glycerol monostearate	酯类Mononitrate
16	麦芽糖Maltose	糖类Carbohydrate	42	甘油Glycerol	酯类Mononitrate
17	景天庚酮糖Sedoheptulose	糖类Carbohydrate	43	2，6-二羟基苯甲酸甲酯 Methyl 2，6-dihydroxybenzoate	酯类Mononitrate
18	蔗糖Sucrose	糖类Carbohydrate	44	三氨基甲酸酯Triscarbamate	酯类Mononitrate
19	环己六醇Inositol	烷烃Alkane	45	尿素Urea	其他Others
20	3-乙基-3-甲基庚烷 3-Ethyl-3-methylheptane	烷烃Alkane	46	茜素Alizarin	其他Others
21	2，2，4，4，6，8，8-庚甲基-壬烷 Nonane，2，2，4，4，6，8，8-heptamethyl-	烷烃Alkane	47	双酚A单甲醚 Bisphenol A monomethyl ether	其他Others
22	3，8-二甲基-十一烷 Undecane，3，8-dimethyl-	烷烃Alkane	48	尸胺Cadaverine	其他Others
23	2，3，4-三羟基丁酸 2，3，4-Trihydroxybutyric acid	有机酸Organic acids	49	二羟蒽醌Danthron	其他Others
24	4-氨基丁酸 4-Aminobutanoic acid	有机酸Organic acids	50	乙醇胺Ethanolamine	其他Others
25	4-羟基苯甲酸 4-Hydroxybenzoic acid	有机酸Organic acids	51	乙基-α-D-吡喃葡萄糖苷 Ethyl-α-D-glucopyranoside	其他Others

Table 1

Fig.1

Fig. 2

Fig.3

Table 2

Fig.4

Fig.5

References 36

1	张志翔.树木学：北方本［M］.2版.北京：中国林业出版社，2008：550.
	ZHANG Z X.Dendrology：North edition［M］.2nd ed.Beijing：China Forestry Publishing House，2008：550.
2	胡萌晓，翟悦，杨淞淇，等.‘鲁赫’刺蔷薇籽成分分析及抗氧化、抗炎活性研究［J］.中国林副特产，2022（1）：1-4，14.
	HU M X， ZHAI Y， YANG S Q，et al.Analysis of composition，anti-oxidation and anti-inflammatory activities of Rosa acicularis ‘Luhe’ seed［J］.Forest by-Product and Speciality in China，2022（1）：1-4，14.
3	冯亚磊，何培，王生贺，等.低温逆境下“鲁赫”刺蔷薇抗性分析［J］.齐齐哈尔大学学报（自然科学版），2017，33（5）：56-63.
	FENG Y L， HE P， WANG S H，et al.The resistance ability of Rosa acicularis ‘Luhe’ under chilling stress［J］.Journal of Qiqihar University（Natural Science Edition），2017，33（5）：56-63.
4	徐宁伟，李津，郭振清，等.白花刺蔷薇种子和果实的形态与组分［J］.河北科技师范学院学报，2011，25（3）：39-43.
	XU N W， LI J， GUO Z Q，et al.The study on the morphological characters and the component analysis of seeds and fruit of Rosa acicularis ［J］.Journal of Hebei Normal University of Science & Technology，2011，25（3）：39-43.
5	韩竞，金哲雄.刺玫果的药理作用及临床应用概况［J］.黑龙江医药，2014，27（1）：106-108.
	HAN J， JIN Z X. Rosa acicularis pharmacological functions and clinical applications［J］.Heilongjiang Medicine Journal，2014，27（1）：106-108.
6	赵梦雅，赵玉红.‘鲁赫’与野生刺蔷薇花精油成分比较［J］.现代食品科技，2019，35（3）：241-248，14.
	ZHAO M Y， ZHAO Y H.Analysis and comparison of essential oil composition between Rosa acicularis ‘Luhe’ and lindl［J］.Modern Food Science and Technology，2019，35（3）：241-248，14.
7	赵玉红，师帅帅，张立钢.‘鲁赫’刺蔷薇叶不同溶剂提取物的活性成分及抗氧化性比较［J］.现代食品科技，2019，35（5）：159-166.
	ZHAO Y H， SHI S S， ZHANG L G.Bioactive compounds extracted by different solvents from Rosa acicularis ‘Luhe’ leaves and their antioxidant activity［J］.Modern Food Science and Technology，2019，35（5）：159-166.
8	WANG Y X， GUO X， ZHANG L G，et al.Effects of deproteinization on rheological properties of polysaccharides from Rosa acicularis ‘Luhe’ and Rosa acicularis Lindl fruits［J］.Journal of Food Measurement and Characterization，2021，15（3）：2500-2515.
9	毕诗畦，穆立蔷.‘鲁赫’刺蔷薇对铅、镉及其混合胁迫的生理响应［J］.西北林学院学报，2018，33（6）：47-55.
	BI S Q， MU L Q.Physiological response of Rosa acicularis ‘Luhe’ to lead，cadmium and mixed stresses［J］.Journal of Northwest Forestry University，2018，33（6）：47-55.
10	易照勤，初振欢，王昊天，等.‘鲁赫’刺蔷薇播种繁殖技术研究［J］.高师理科学刊，2017，37（4）：47-51.
	YI Z Q， CHU Z H， WANG H T，et al.Study on seed propagation technique of Rosa acicularis ‘Luhe’［J］.Journal of Science of Teachers’ College and University，2017，37（4）：47-51.
11	FIEHN O， KOPKA J， DÖRMANN P，et al.Metabolite profiling for plant functional genomics［J］.Nature Biotechnology，2000，18（11）：1157-1161.
12	NICHOLSON J K， LINDON J C， HOLMES E.'Metabonomics'：understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data［J］.Xenobiotica，1999，29（11）：1181-1189.
13	DIXON R A， STRACK D.Phytochemistry meets genome analysis，and beyond［J］.Phytochemistry，2003，62（6）：815-816.
14	漆小泉，王玉兰，陈晓亚.植物代谢组学：方法与应用［M］.北京：化学工业出版社，2011：304.
	QI X Q， WANG Y L， CHEN X Y.Plant metabolomics：methods and applications［M］.Beijing：Chemical Industry Press，2011：304.
15	卫晶晶.金叶弯刺蔷薇叶片形态、叶绿素生物合成及光合作用特性的研究［D］.北京：中国农业科学院，2013.
	WEI J J.Leaf morphological ultrastructure，chlorophyll biosynthesis and photosynthesis of the yellow-leaf mutant of Rosa beggeriana ［D］.Beijing：Chinese Academy of Agricultural Sciences，2013.
16	刘龙桀，吴可心，刁云飞，等.基于GC-MS分析东北红豆杉野生种与栽培种的代谢差异［J］.植物研究，2021，41（5）：798-806.
	LIU L J， WU K X， DIAO Y F，et al.Metabolic differences between wild and cultivated species of Taxus cuspidata based on GC-MS［J］.Bulletin of Botanical Research，2021，41（5）：798-806.
17	LIU Y， LIU J， WU K X，et al.A rapid method for sensitive profiling of bioactive triterpene and flavonoid from Astragalus mongholicus and Astragalus membranaceus by ultra-pressure liquid chromatography with tandem mass spectrometry［J］.Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences，2018，1085：110-118.
18	刘佳.运用代谢组和转录组解析人参皂苷组织和发育特异性的代谢差异［D］.哈尔滨：东北林业大学，2017.
	LIU J.The dissection of ginsenoside metabolic differences controlled by tissue，development and species through metabolomics and transcriptomic approaches［D］.Harbin：Northeast Forestry University，2017.
19	刘洋.应用代谢组学对比研究两种黄芪响应UV-B和干旱胁迫的代谢基础［D］.哈尔滨：东北林业大学，2018.
	LIU Y.Comparative metabolomics study on the metabolic basis of two varieties of Astragalus in response to UV-B and drought stress［D］.Harbin：Northeast Forestry University，2018.
20	ALVAREZ S， NALDRETT M J.Mass spectrometry based untargeted metabolomics for plant systems biology［J］.Emerging Topics in Life Sciences，2021，5（2）：189-201.
21	YOUNG R F， COY D L， FEDORAK P M.Evaluating MTBSTFA derivatization reagents for measuring naphthenic acids by gas chromatography-mass spectrometry［J］.Analytical Methods，2010，2（6）：765.
22	BLAU K， HALKET J M.Handbook of derivatives for chromatography：2nd ed［M］.Heyden，1993：369.
23	姜海洋.马铃薯块茎形成期糖类物质代谢机制与产量形成的研究［D］.大庆：黑龙江八一农垦大学，2017.
	JIANG H Y.Studies on carbohydrate metabolism yield during the formation of potato tuber［D］.Daqing：Heilongjiang Bayi Agricultural University，2017.
24	赵艳玲，王梅.EuDQD/SDH6基因在巨尾桉莽草酸途径中的功能分析［J/OL］.分子植物育种.［2022-06-12］..
	ZHAO Y L， WANG M.The functional study of shikimate dehydrogenases genes EuDQD/SDH6 in Eucalyptus grandios×E.urophylla ［J］.Molecular Plant Breeding.［2022-06-12］..
25	FRANÇA S C， ROBERTO P G， MARINS M A，et al.Biosynthesis of secondary metabolites in sugarcane［J］.Genetics and Molecular Biology，2001，24（1/2/3/4）：243-250.
26	HOŠŤÁLEK Z.Regulation of gene expression in secondary biosynthesis［J］.Regulation of Secondary Product and Plant Hormone Metabolism，1979：111-120.
27	李玲，孙宇婷，任杏，等.不同生长期薰衣草枝叶功能性状及其与环境因子的相关性［J］.东北林业大学学报，2021，49（12）：21-28.
	LI L， SUN Y T， REN X，et al.Functional traits of branches and leaves，and their correlation with environmental factors for Lavandula angustifolia Mill.in different growth stages［J］.Journal of Northeast Forestry University，2021，49（12）：21-28.
28	SATISH L， SHAMILI S， YOLCU S，et al.Biosynthesis of secondary metabolites in plants as influenced by different factors［M］.Plant-derived Bioactives，2020：61-100.
29	谢小东，程廷才，王根洪，等.植物ABC和MATE转运蛋白与次生代谢物跨膜转运［J］.植物生理学报，2011，47（8）：752-758.
	XIE X D， CHENG T C， WANG G H，et al.ABC and MATE transporters of plant and their roles in membrane transport of secondary metabolites［J］.Plant Physiology Journal，2011，47（8）：752-758.
30	何俊瑜，顾津羽，胡春梅，等.水杨酸与硝普钠对采后芒果果实炭疽病抗性和苯丙烷代谢的协同诱导效应［J］.食品科学，2022，43（3）：178-186.
	HE J Y， GU J Y， HU C M，et al.Synergistic effect of combined salicylic acid and nitric oxide in anthracnose resistance and phenylpropanoid metabolism in mango fruit［J］.Food Science，2022，43（3）：178-186.
31	COLQUHOUN T A， KIM J Y， WEDDE A E，et al. PhMYB4 fine-tunes the floral volatile signature of Petunia×hybrida through PhC4H ［J］.Journal of Experimental Botany，2011，62（3）：1133-1143.
32	袁婷婷，董艳，赵骞.苯甲酸对蚕豆枯萎病菌致病力及根系组织结构抗性的影响［J］.植物生理学报，2020，56（3）：441-454.
	YUAN T T， DONG Y， ZHAO Q.Effects of benzoic acid on pathogenicity of Fusarium oxysporum f.sp.fabae and tissue structure resistance of faba bean root［J］.Plant Physiology Journal，2020，56（3）：441-454.
33	HSUEH M T， FAN C， LO H F，et al.Effects of light and autotoxicity on the reproduction of Bidens pilosa L.：from laboratory to the field［J］.Agriculture，2020，10（11）：555.
34	郭伟，薛盈文，于崧，等.邻苯二甲酸与对羟基苯甲酸对燕麦幼苗生长及抗氧化特性的影响［J］.植物生理学报，2017，53（10）：1885-1892.
	GUO W， XUE Y W， YU S，et al.Effects of phthalic acid and p-hydroxybenzonic acid on seedling growth and antioxidant properties of oat（Avena nuda）［J］.Plant Physiology Journal，2017，53（10）：1885-1892.
35	LESS H， GALILI G.Principal transcriptional programs regulating plant amino acid metabolism in response to abiotic stresses［J］.Plant Physiology，2008，147（1）：316-330.
36	GHOSH S， CHISTI Y， BANERJEE U C.Production of shikimic acid［J］.Biotechnology Advances，2012，30（6）：1425-1431.

名称 Name	VIP	FC	log₂FC	P	结果 Results
β-龙胆二糖beta-Gentiobiose	1.081 25	19.674 100	4.298 226	0.000 946	下调Down
2，3，4-三羟基丁酸 2，3，4-Trihydroxybutyric acid	1.522 94	4.287 685	2.100 199	0.000 719	下调Down
阿拉伯呋喃糖Arabinofuranose	1.509 22	2.242 054	1.164 821	0.008 822	下调Down
乙醇胺Ethanolamine	1.415 92	1.150 863	0.202 716	0.000 334	下调Down
乙基-α-D-吡喃葡萄糖苷 Ethyl-α-D-glucopyranoside	1.649 04	2.855 704	1.513 846	0.001 445	下调Down
没食子酸Gallic acid	1.543 46	4.828 324	2.271 523	0.006 287	下调Down
甘油Glycerol	1.204 64	53.930 700	5.753 035	0.001 827	下调Down
乳酸Lactic acid	1.147 82	3.787 665	1.921 309	0.000 568	下调Down
苹果酸Malic acid	1.586 60	4.304 292	2.105 776	0.004 562	下调Down
麦芽糖Maltose	1.664 90	6.913 381	2.789 391	0.001 095	下调Down
硬脂酸Stearic acid	1.652 46	1.554 813	0.636 741	0.006 598	下调Down
3，8-二甲基-十一烷 Undecane，3，8-dimethyl-	1.541 09	1.158 301	0.212 010	0.000 412	下调Down
4-氨基丁酸 4-Aminobutanoic acid	1.084 23	0.378 054	-1.403 340	0.002 072	上调Down
4-羟基苯甲酸 4-Hydroxybenzoic acid	1.506 89	0.706 741	-0.500 750	0.000 326	上调Up
双酚A单甲醚 Bisphenol A monomethyl ether	1.516 88	0.518 734	-0.946 930	0.027 575	上调Up
绿原酸Chlorogenic acid	1.243 60	0.454 291	-1.138 310	0.014 054	上调Up
L-丙氨酸L-Alanine	1.349 34	0.229 774	-2.121 720	0.001 650	上调Up
奎宁酸Quininic acid	1.249 88	0.040 110	-4.639 890	0.047 114	上调Up
莽草酸Shikimic acid	1.668 16	0.184 006	-2.442 180	0.010 847	上调Up
缬氨酸Valine	1.517 29	0.951 894	-0.071 130	0.000 219	上调Up

[1]	Wanting JIANG, Nan YANG, Chen CHEN, Xiaorui GUO, Zhonghua TANG, Hongzheng WANG. Effects of Selenium Treatment on Physiology and Primary Metabolism of Astragalus adsurgens [J]. Bulletin of Botanical Research, 2022, 42(6): 1062-1069.
[2]	Pengying CHEN, Chan YANG, Jian LUO. Diversity of Wild Poisonous Plant Resources in Namjagbarwa Region [J]. Bulletin of Botanical Research, 2022, 42(6): 921-929.
[3]	Ermei CHANG, Jianfeng LIU, Yuening HUANG, Hongli LI, Bingyan SHAN, Zeping JIANG, Xiulian ZHAO. Comparison of Genetic Diversity Between Wild and ex-situ Conservation Populations of Cupressus chengiana [J]. Bulletin of Botanical Research, 2022, 42(5): 772-779.
[4]	Si-Han WANG, Tao YANG, Jin-Zhu ZHANG, Jie DONG, Zhi-Ling KOU, Dai-Di CHE. Effects of Changes in Seed Cell Tissue Structure and Endogenous Hormones on Dormancy of Wild Rosa rugosa Fruit during Development [J]. Bulletin of Botanical Research, 2021, 41(3): 387-394.
[5]	Guo-Lin FU, Ting-Ting LI, Ye-Hua ZHANG, Xiang-Ying WEN, Xue-Chu MA, Chu-Ping WU. Phenological Observation of the Extremely Small Population Plant Styrax zhejiangensis [J]. Bulletin of Botanical Research, 2021, 41(2): 168-173.
[6]	YE Xing-Zhuang, LIU Dan, LUO Jia-Jia, FAN Hui-Hua, ZHANG Guo-Fang, LIU Bao, CHEN Shi-Pin. Transcriptome Analysis for Rare and Endangered Plants of Semiliquidambar cathayensis [J]. Bulletin of Botanical Research, 2019, 39(2): 276-286.
[7]	DU Hui-Cong, ZHANG Ying, TIAN Min, WANG Cai-Xia. Study on The Seed Biological Characteristics of Chimonanthus praecox of Zhejiang Province [J]. Bulletin of Botanical Research, 2018, 38(1): 7-13.
[8]	ZHANG Yin-Bo;ZHANG Xiao-Long;LU Yi-Meng;LI Ming. Resource and Floristic Characteristics of the Key Protection Wild Plants in Shanxi [J]. Bulletin of Botanical Research, 2013, 33(1): 18-23.
[9]	WANG Jie;YANG Zhi-Ling;YANG Xu. Analysis on the Aroma Components of Different Flower Parts in Magnolia officinalis* Wild and Cultivated Species [J]. Bulletin of Botanical Research, 2012, 32(2): 237-242.
[10]	QIAN Gang;WANG Da-Zhong;LUO Su-Yuan;TANG Xian-Chun. Assessment of Genetic Diversity in Guizhou Wild Barley Using SSR Markers Ralated to Malting Quality [J]. Bulletin of Botanical Research, 2009, 29(6): 696-700.
[11]	CHENG Zhuan-Hong;ZHAO Shu-Lan;DUO Li-An*. Physiological and Ecological Responses of 3 Wild Groundcover Plants to Traffic Stress [J]. Bulletin of Botanical Research, 2008, 28(5): 614-617.
[12]	YANG Li-Ping. STUDY ON ANTHESIS FLAWERING AND FERTILITY OF LILIUM CONCOLOR SALISB. VAR PULCHELLUM (FISCH) REGEL [J]. Bulletin of Botanical Research, 2000, 20(4): 389-394.
[13]	Liu Qi-jing, Wang He-xin, YangXiu-qing. STUDIES ON TYPICAL PRIMITIVE BROAD-LEAVED PINUS KORAIENSIS FOREST COMMUNITY IN WHICH WILD GINSENG DISTRIBUTES NATURALLY AND ITS CHARACTERISTICS [J]. Bulletin of Botanical Research, 1994, 14(3): 292-298.