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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (3): 352-357.doi: 10.7525/j.issn.1673-5102.2022.03.004

• Systematic and Evolutionary • Previous Articles     Next Articles

Yandong GUO1, Mingxia ZHANG1, Yifeng ZHOU1, Xiling DAI2, Quanxi WANG2, Jianguo CAO2()   

  1. 1.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014
    2.College of Life Science,Shanghai Normal University,Shanghai 200234
  • Received:2021-06-23 Online:2022-05-20 Published:2022-05-20
  • Contact: Jianguo CAO E-mail:cao101@5hnu.edu.cn
  • About author:GUO Yandong(1990—),female,master,major in reproductive and developmental biology of ferns.
  • Supported by:
    National Natural Science Foundation of China(30970267);Key Project of Shanghai Municipal Education Commission(12ZZ128)


The oogenesis of Lygodium japonicum(Thunb.) Sw. was studied by histochemical method in this study. The results of PAS reaction showed that the extracellular viscous substances of neck canal and ventral canal cells were polysaccharides; there were plastids and rich starch granules in cell of archegonium in the early stage of oogenesis, with the development of archegonium, the number and volume of starch granules in the egg and neck canal and ventral canal cells gradually decreased, and finally degenerated and disappeared. The results of Sudan black B reaction showed that the extracellular viscous substances of neck canal and ventral canal cells of L. japonicum also contain lipids, but there was no obvious lipids production in egg cells. Comprehensive studies showed that there was no accumulation of carbohydrates or lipids in the plasma membrane of egg cells. From the perspective of histochemistry, this study confirmed the particularity of L. japonicum, it had the characteristics of both primitive ferns and evolutionary ferns.

Key words: Lygodium japonicum(Thunb.) Sw., oogenesis, histochemistry, PAS reaction, Sudan black B reaction

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