Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (3): 352-357.doi: 10.7525/j.issn.1673-5102.2022.03.004
• Systematic and Evolutionary •
Yandong GUO1, Mingxia ZHANG1, Yifeng ZHOU1, Xiling DAI2, Quanxi WANG2, Jianguo CAO2()
The oogenesis of Lygodium japonicum（Thunb.） Sw. was studied by histochemical method in this study. The results of PAS reaction showed that the extracellular viscous substances of neck canal and ventral canal cells were polysaccharides； there were plastids and rich starch granules in cell of archegonium in the early stage of oogenesis， with the development of archegonium， the number and volume of starch granules in the egg and neck canal and ventral canal cells gradually decreased， and finally degenerated and disappeared. The results of Sudan black B reaction showed that the extracellular viscous substances of neck canal and ventral canal cells of L. japonicum also contain lipids， but there was no obvious lipids production in egg cells. Comprehensive studies showed that there was no accumulation of carbohydrates or lipids in the plasma membrane of egg cells. From the perspective of histochemistry， this study confirmed the particularity of L. japonicum， it had the characteristics of both primitive ferns and evolutionary ferns.
Lygodium japonicum（Thunb.） Sw.,
Sudan black B reaction
Yandong GUO, Mingxia ZHANG, Yifeng ZHOU, Xiling DAI, Quanxi WANG, Jianguo CAO. [J]. Bulletin of Botanical Research, 2022, 42(3): 352-357.
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PAS reaction in the oogenesis of L. japonicum（Thunb.） Sw.1.Initial cell period；2-3.Primary cell period；4-5.Central cell period；6.Young egg period；7-11.Developmental egg period；12-14.Mature egg period
Plate Ⅱ Sudan black B reaction in the oogenesis of L. japonicum（Thunb.） Sw.1.Primary cell period；2.Central cell period；3.Young egg period；4-7.Developmental egg period；8-9.Mature egg period