Megasporogenesis and Microsporogenesis and Development of Female and Male Gametophytes in Mutants lpls with Long Pistil and Long Stamen from Fagopyrum esculentum Moench

doi:10.7525/j.issn.1673-5102.2021.06.010

Abstract

Abstract:

The Mega-and Micro-sporogenesis and development of female and male gametophytes in mutant Buckwheat with long pistil and long stamen were investigated by the paraffin section. The results showed that anthers were tetradymous and the anther wall was composed of four layers of cells. The meiosis of microspore mother cells was followed by simultaneous cytokinesis and resulted in tetrahedral tetrads. A few microspores with two nuclei were observed in tetrahedral tetrads. Most of mature pollen grains were 2-cell type， while a few 3-cell mature pollen grains were also observed in anther sac. Orthotropous and bi-integument ovule， as well as thick nucellus was found in embryo sac. The megaspore mother cells underwent meiosis and were divided into liner type tetrads， and the megaspore in the chalazal end was functional one. The embryo sac was Polygonum type. The stamen primordia emerged before the appearance of gynoecium primordia. Single or tow-nucellus microspores were released from tetrahedral tetrads when the forth leaf arose from the main stem， and the mature pollen grains were observed when the seventh leaf arose from the main stem. Megaspore mother cells emerged when the tetrahedral tetrads formed， while the functional megaspore appeared until 2-cell pollen grains formed. 2-cell and 3-cell mature pollens coexisted after development of the seventh leaf on the main stem， while the mature embryo sac with 8 nucellus and 7 cells resulted from continuous mitosis of the functional megaspore.

Key words: Fagopyrum esculentum, long pistil and long stamen, mega-and microsporogenesis, female and male gametophytes

CLC Number:

S517

Xuan WANG, Zhi-Xiong LIU. Megasporogenesis and Microsporogenesis and Development of Female and Male Gametophytes in Mutants lpls with Long Pistil and Long Stamen from Fagopyrum esculentum Moench[J]. Bulletin of Botanical Research, 2021, 41(6): 921-927.

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