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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (6): 948-955.doi: 10.7525/j.issn.1673-5102.2018.06.019

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Effect of Fungal Elicitor on Gallic Acid Accumulation in the Cells of Acer ginnala Maxim

QI Feng-Hui1,2, CHEN Si-Qi1, JING Tian-Zhong3, ZHANG Yu-Xin1, ZHAN Ya-Guang1,2   

  1. 1. College of Life Sciences, Northeast Forestry University, Harbin 150040;
    2. State Key of Laboratry of Tree Breeding(NEFU), Harbin 150040;
    3. School of Forestry, Northeast Forestry University, Harbin 150040
  • Received:2018-06-05 Online:2018-11-15 Published:2018-11-01
  • Supported by:
    This work was supported by Fundamental Research Funds for the Central Universities(DL13CA07);Project of research foundation of the talent of scientific and technical innovation of Harbin City(2013RFQXJ026)

Abstract: The leaf of Acer ginnala Maxim. contains gallic aicd, however, in a low content. The fungal elicitors increase some secondary metabolites in plant. However, the mechanism of the increase effect are not well studied. A fungal elicitor prepared with Phomopsis sp. was added during the logarithmic growth phase of suspension cultures of A.ginnala cells. As a result, the accumulation of gallic acid increased after 24 h and reached the peak (12.2 mg·g-1DW, 1.58-fold of control) at 48 h. Defensive responses of the cell to the endophytical elicitor were different from those to a pathogen or an abiotic stress. The endophytical elicitor did not induce medium alkalinization and an internal influx of calcium ions, but increased the permeability of plant plasma membrane. The medium conductivity increased and the nuclei division was observed, which indicate that the fungal elicitor promotes mitosis of the plant cells, which consumes more mineral nutrients to grow. Compared to the control, the activity of phenylalaninammo-nialyase (PAL) increased up to 1.75 times, indicating the accumulation of GA can be attributed to the rise of PAL activity.

Key words: Acer ginnala Maxim., fungal elicitor, gallic acid, suspension culture

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