The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2

doi:10.7525/j.issn.1673-5102.2017.06.018

Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (6): 941-946.doi: 10.7525/j.issn.1673-5102.2017.06.018

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The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2

YANG Ming-Fei¹, ZHANG Jing³, SU Wen², WANG Peng³, ZHANG Sun-Dong³, KOU Ping³, JIAO Jiao³, YANG Yu-Chun⁴, FU Yu-Jie³

1. Research and Development Department of Kitanihon Pharmaceutical CO., No. 55, Kamiichi, Nakaniikawa District, Toyama, Japan 930-0314;
2. Management Center of Public Medical and Health Institutions in Doumen District, Zhuhai City, Guangdong Province, Zhuhai 519125;
3. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
4. Forestry Academy of Jilin Province, Changchun 130033

Received:2017-03-15 Online:2017-11-15 Published:2017-11-25
Supported by:
"13th Five-Year" The National Key Research Projects(2016YFD0600805)

Abstract

Abstract: As the single active ingredient is poor and prone to drug resistance on effects of lose weight and lipid-lowering, so the study focuses on screening and optimizing the optimal concentration ratio of pinoleic acid/L-carnitine to reduce the lipid of HepG2 cell, which provides the basic data for the development of pinoleic acid lipid-lowering products. Oleic acid induced HepG2 cell adipogenesis, which was used to simulate the model of nonalcoholic fatty liver in vitro. The optimal concentration in combination of pinoleic acid/L-Carnitine decrease lipid was determinated by oil red O staining. The results show that the optimum concentration of oleic acid-induced HepG2 cell steatosis is 0.5 mmol·L^-1.The optimal lipolysis concentration of pinoleic acid is 6.25 μmol·L^-1. The optimal lipid metabolism concentration of L-Carnitine is 250 μmol·L^-1. The best lipid-lowering ratio of pinoleic acid/L-carnitine(PLA/LC) is 1:40(μmol·L^-1/μmol·L^-1). The characteristics of pinoleic acid and L-Carnitine complexes decreased the doses and improved synergistic effect, Therefore, Combination of pinoleic acid/L-Carnitine has a potential prospectin in the development of lipid-lowering diet products.

Key words: pinoleic acid, L-Carnitine, complexes, lipid-lowering, nonalcoholic fatty liver disease

CLC Number:

Q547

YANG Ming-Fei, ZHANG Jing, SU Wen, WANG Peng, ZHANG Sun-Dong, KOU Ping, JIAO Jiao, YANG Yu-Chun, FU Yu-Jie. The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2[J]. Bulletin of Botanical Research, 2017, 37(6): 941-946.

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The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2

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