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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (5): 690-699.doi: 10.7525/j.issn.1673-5102.2017.05.008

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Study on Rapid Seedling-raising Technology of Tree Peony Embryo Culture

XU Li, CHENG Fang-Yun, ZHONG Yuan   

  1. College of Landscape Architecture, Beijing Forestry, National Flower Engineering Technology Research Center, Beijing 100083
  • Received:2017-03-27 Online:2017-09-15 Published:2017-09-15
  • Supported by:
    Beijing supports the common construction project in central University(2016GJ-02)

Abstract: This study used Paeonia ostii ‘Fengdan’ and Paeonia rockii as explants, established efficient method of Seedling-raising of tree peony embryo culture through the study of hormones, light conditions, medium ingredients and activated carbon(AC) on the in vitro embryo culture. The main result:In vitro embryos radicle germination in 10 d, grow true leaves in 20 d, root system developed in 25 d, transplanting in 40 d, after 3 months the seedlings grow well in the medium of improved MS(calcium doubled)+0.2 g·L-1AC+0.5 mg·L-1GA+0.5 mg·L-1BA. Suitable medium for ‘Fengdan’:improved MS(calcium doubled,macroelement doubled)+0.6 g·L-1AC+1.0 mg·L-1GA; medium for P.rockii:improved MS(calcium doubled,macroelement doubled)+0.6 g·L-1 AC+0.5 mg·L-1GA; The seedling rate of ‘Fengdan’ was 63.88%, the survival rate was 66.34%, and the rate of seedling of P.rockii was 93.75%, and survival rates vary among breeds, with a maximum of 100%.

Key words: embryo culture, Paeonia ostii ‘Fengdan’, Paeonia rockii, activated carbon

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