Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (2): 245-251.doi: 10.7525/j.issn.1673-5102.2014.02.017
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WANG Xu-Hui;QIAO Kun;WANG Zhen-Juan;GUAN Qing-Jie*;LIU Shen-Kui
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Abstract: Cyclophilins (CyPs) are widely distributed in organisms, and play important roles. Cyclophilin A-like was screened in our study from Chlorella sp. cDNA library. By extracting Arabidopsis thaliana protoplasts that was transformed the 35S::CyPA::GFP fusion plasmid, it showed that CyPA located in the chloroplasts. Overexpression of CyPA gene in yeast enhanced the InV yeast growth ability to resistance of NaCl, NaHCO3 and drought stress. Real-time quantitative PCR showed that under abiotic stress conditions, CyPA gene expression quantity increased. So we speculated that CyPA genes were associated with abiotic stress resistance, through the cyclophilin A involved in improving survival ability of resistant organisms.
Key words: chlorella, cyclophilins, real time quantitative PCR, expression pattern of gene
CLC Number:
Q51
WANG Xu-Hui;QIAO Kun;WANG Zhen-Juan;GUAN Qing-Jie*;LIU Shen-Kui. Cloning and Expression Analysis of Cyclophilins A(CyPA) Gene form Chlorella[J]. Bulletin of Botanical Research, 2014, 34(2): 245-251.
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URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2014.02.017
https://bbr.nefu.edu.cn/EN/Y2014/V34/I2/245