Establishment of RAPD reaction system on the moss

Bulletin of Botanical Research ›› 2004, Vol. 24 ›› Issue (4): 482-485.

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Establishment of RAPD reaction system on the moss

SHA Wei¹, LI Jing¹, CAO Tong², SU Xiao-Ming¹

1. Department of Biology, College of Life Science and Engineering, Qiqihar University, Qiqihar 161006;
2. Department of Biology, Shanghai Normal University, Shanghai 200234

Received:2004-02-09 Online:2004-12-15 Published:2016-06-14

Abstract

Abstract: To obtain the optimum random amplified polymorphic DNA (RAPD)reaction system, one test was designed to screen the suitable concentration of 4 factors (Taq, dNTP, primer, TemplateDNA)at 4 levels in the reaction system.According to the results we obtain the optimum concentration of the four factors of moss.On the basis of this we attain 30 polymorphic primers showing genetic differences among moss were screened from 100 random primers.

Key words: moss, random amplified polymorphic DNA, random primers, genetic differences

SHA Wei, LI Jing, CAO Tong, SU Xiao-Ming . Establishment of RAPD reaction system on the moss[J]. Bulletin of Botanical Research, 2004, 24(4): 482-485.

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Establishment of RAPD reaction system on the moss

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