Abstract: The leaves of Actinidia arguta Planch were cultured on MS basic med-ium supplemented with 2.4-D or NAA and zeatin in vitro. The callus wereinduced successfullg. The results have been obtained as follows:the callus fitting for the establishment of cell line coulb be obtained from leaves of Act-inidia arguta Planch on MS+ZT1mg/l+2.4-D3mg/l. Tbe highest cell surv-ival rate has been obtained from callus that had been cultured for ten days. The satisfied result have beenobtained on MS+ZT1mg+2.4-D3mg/l.The high-est frequency of round cell is medium with 200mg/l myo-inositol. Suitable concentration of sucrose is 5%.