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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (6): 952-956.doi: 10.7525/j.issn.1673-5102.2015.06.025

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Rapid Identification of Blumea balsamifera by Specific PCR and Fluorescence Detection

ZHAO Dan1;ZHOU Tao1*;YUAN Yuan2;XIAO Cheng-Hong1;JIANG Wei-Ke1;KANG Chuan-Zhi1,2   

  1. 1.Guiyang College of Traditional Chinese Medicine,Guiyang 550025;
    2.State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700
  • Online:2015-11-20 Published:2016-01-18
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Abstract: The experiment was conducted to establish a rapid and reliable molecular identification method for Blumea balsamifera DC. By genomic DNA extraction method, we screened B.balsamifera and its adulterants, designed primers with the specific loci of B.balsamifera, and optimized the amplification conditions of PCR. We detected the PCR products by fluorescence reaction. The alkaline lysis method of extracting genomic DNA was more appropriate for B.balsamifera. It could specifically amplified by primers of tDNA, and its products were green fluorescent by fluorescence detection. While, its adulterants were non-reaction occurring. This method simplified operation steps and saved time with accurate result, and it could be served as identification method for B.balsamifera.

Key words: Blumea balsamifera DC., specific PCR, fluorescence detection

CLC Number: