Abstract: In this paper, the spores of Dryopteris crassirhizoma after three years of low-temperature storage were used as the experimental material, the influences of centrifugal, disinfection, culture media and light quality on spore germination were respectively studied. The results showed that centrifugal (≤14 000 r·min^-1, ≤30 min) almost had no influence on spore germination; Sterilizing spore surface for 10-20 min with 1% NaClO was the best sterile method; Modified Knop’s medium was the best for spore germination; Spore could not germinate in dark, but the dark treatment could improve the regularity of spore germination; Red light could promote spore germinating about 1 day earlier than white light, but had no obvious influence on promoting spore germination rate.

Key words: Dryopteris crassirhizoma, centrifugal, disinfection, culture media, light quality, spore germination