Proteomic Screening and RcBSK7 Resistance of Ricinus communis under Cadmium(Cd) Stress

doi:10.7525/j.issn.1673-5102.2023.01.005

Abstract

Abstract:

In order to reveal the mechanism of Ricinus communis response to heavy metal cadmium（Cd） stress， and screen out the resistance genes involved in Cd stress， by observing the seed germination and plant growth status， castor plants treated with water were used as controls to study their effects under three doses（300， 700 and 1 000 mg·L^-1） of Cd stress. The response mechanism is expected to provide new ideas for revealing the defense and detoxification mechanism of castor plant under in Cd stress. Using differential proteomics to analyze the network regulation mechanism of castor under Cd stress， that is， with the increase of Cd stress concentration， the castor plant blocks the absorption of heavy metal Cd by roots， improves their own antioxidant capacity， and inhibits Cd²⁺ operation. As well as the induction of programmed cell death and other detoxification processes to resist Cd stress damage； according to the results of omics analysis， a significantly different gene RcBSK7 was screened. Through functional verification in Arabidopsis， it can be seen that this gene can improve tolerance of castor plants to Cd stress. This study enhances the understanding of the diversity and complexity of castor plants under three Cd stresses， and provides a valuable theoretical basis for the identification of Cd-tolerant genes and the restoration of heavy metal pollution in the soil.

Key words: Ricinus communis, heavy metal Cd, differential proteomics, serine/threonine protein kinase, soil pollution remediation

CLC Number:

Q344+.13

Huibo ZHAO, Zhiqiang ZHAO, Chunguang BAO, Qi WEN, Ruxin LI, Fenglan HUANG. Proteomic Screening and RcBSK7 Resistance of Ricinus communis under Cadmium(Cd) Stress[J]. Bulletin of Botanical Research, 2023, 43(1): 36-50.

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名称 Name	引物序列（5′→3′） Primer sequence（5′→3′）	退火温度 Tm /℃
Actin-R	GTGCTTGATTCTGGTGATGGC	56.9
Actin-F	TTGGCAGTCTCAAGTTCTTGCTC	57.5
B9RCB9-R	GGTGAGGCTTCCTTAAGAATGG	55.7
B9RCB9-F	CCTGCATCTGATCAGTCCAC	55.6
B9T1G7-R	GCTGCAATCCTGGTTCCAG	55.9
B9T1G7-F	GTCACTGGCAGTGATTCTCC	57.0
B9RXH6-R	GACTCGTGGAGTTAGCATG	52.7
B9RXH6-F	CGATCTTGTGTCTGATTCTCC	52.9
B9RTU8-R	GGTACATGTTAGCACTAGCGG	55.6
B9RTU8-F	CTCTAACATGAATAATGTACCGC	55.2
B9SIQ2-R	GACAGCCGTGCCTCAATTTC	57.2
B9SIQ2-F	GGATACGGCCATGAGTAGGC	58.3
B9SR47-R	GAAGCGTCAGGTCATGATTAGG	55.5
B9SR47-F	CAATCTCCCATTTAACTCGACCAC	55.6
B9RWI8-R	GCGCACTATTCGTGGGATGATTC	58.7
B9RWI8-F	CCTTGAGCATCAGGGATAAC	58.5
B9RGI8-R	GCACTGTGCTTTGTGTCCATG	57.5
B9RGI8-F	CAGGATCACTCCACATTAGATCGC	57.5
B9T3N2-R	GTGCGTGACAGTATGAGGATG	55.8
B9T3N2-F	GCAAACCTCTATGCCAACAC	55.1

Cd质量浓度 Concentration /（mg·L^-1）	鲜质量 Fresh weight /g	干质量 Dry weight /g	主根长 Taproot length /cm	主根粗 Taproot thick /cm	侧根数量 Number of lateral roots
0	52.05±3.26a	10.13±2.56a	23.16±2.01a	2.44±1.12a	24±3a
100	51.11±2.68a	12.54±1.08b	23.21±1.93a	2.61±2.58a	28±5a
200	53.75±2.14a	12.69±2.13b	23.94±2.30a	2.78±0.35b	32±2a
300	57.82±1.02a	10.35±1.92a	24.63±1.12a	2.51±1.52b	28±4a
400	41.32±2.02b	10.51±3.23a	19.23±1.02b	1.95±1.12c	13±3b
500	40.56±3.74b	08.64±1.82c	16.03±0.98c	1.78±1.17c	17±4c
600	38.38±1.22b	6.39±1.96c	19.47±1.01b	1.53±0.85c	09±2d
700	30.65±2.83c	6.79±2.01c	17.03±0.00c	1.20±0.13d	7±2d
800	14.74±1.63d	4.39±1.81d	06.66±1.25d	0.87±0.31e	0±0e
900	14.61±2.05d	4.02±2.21d	06.13±1.25d	0.82±0.69e	0±0e
1 000	12.39±1.05e	3.86±1.04d	04.82±2.01e	0.53±0.24e	0±0e