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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (2): 308-313.doi: 10.7525/j.issn.1673-5102.2020.02.019

• Scientific Notes • Previous Articles     Next Articles

Optimization of Dry Enzymatic Extraction Process and Physicochemical Properties of Pine Seed Oil

ZU Shu-Chong   

  1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2019-10-15 Online:2020-03-05 Published:2020-03-06

Abstract: Used a single factor experiment to optimize a dry enzyme extraction process of pine seed oil. The best preparation process for dry enzymatic extraction of pine seed oil was:0.2% of amylase, the ratio of material to liquid of 6:1, the hydrolysis temperature of 55℃, and the hydrolysis time of 8 h, and the maximum oil yield 90.2% with the residual oil rate of 6.2%. The content of unsaturated fatty acids in pine seed oil extracted after testing for the best preparation conditions was 90.09%, of which oleic acid content was 26.84%, mediating acid content was 2.41%, linoleic acid content was 46.25%, and pinolacol acid was 14.59%. The content of palmitic acid in saturated fatty acids was 6.5%, and the stearic acid was 3.41%. The pine oil had an acid value of 2.61 mg·g-1, a peroxide value of 1.56 mmol·kg-1, and a malondialdehyde content of 0.41 mg·kg-1. All the test results are higher than the requirements of the quality standards in pine seed oil in the "Food Industry Standard of the People's Republic of China LS/T 3242-2014".

Key words: pine seed oil, dry enzymatic method, single factor experiment, pinolenic acid, physical and chemical properties

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