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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (6): 866-872.doi: 10.7525/j.issn.1673-5102.2015.06.013

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Identification of Protein Interactions between Flowering Repressors BpFLC and BpSVP from Betula platyphylla

ZANG Li-Na;ZHENG Tang-Chun;DAI Li-Juan;LIU Cai-Xia;QU Guan-Zheng*   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Online:2015-11-20 Published:2016-01-18
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Abstract: The experiment was conducted to study the mechanism of interaction between SVP and FLC in Betula platyphylla. The truncated genes of BpFLC1-6 and BpSVP1-6 were, respectively, cloned from yeast recombination plasmids pGBKT7-BpFLC and pGBKT7-BpSVP. pGBKT7-BpFLC1-6×pGADT7-BpSVP and pGBKT7-BpFLC×pGADT7-BpSVP1-6 were co-transferred into yeast Y2HGold. The yeast strains of Y2HGold[pGBKT7-BpFLC2-5×pGADT7-BpSVP] could grew on selective agar plates TDO, QTO/A and QDO/X/A with blue stains. BpSVP truncated forms and BpFLC2-5 protein could act with each other to form heterodimers. Then, Y2HGold[pGBKT7-BpFLC×pGADT7-BpSVP2-5] were brought into proximity to form protein compounds and activate transcription of four independent reporter genes of AUR1-C, HIS3, ADE2 and MEL1. The interactions between BpFLC3 and BpSVP3 were tested to confirm the acting domains. The yeast Y2HGold[pGBKT7-BpFLC3×pGADT7-BpSVP3] exhibited blue stains on selective agar plates QDO/X/A. The K domain of BpFLC and that of BpSVP were the key structure domains and mediated the protein interactions between BpFLC and BpSVP.

Key words: Betula platyphylla, BpFLC, BpSVP, yeast two-hybrid, truncated form, protein interaction

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