Abstract: By the method of uniform design, the reaction system for SRAP marker in Rhodomyrtus tomentosa(Ait.) Hassk. was established and optimized. The results showed that an optimal 25 μL reaction system of SRAP for R.tomentosa included 2.5 μL 10×PCR buffer, 1.0 U Taq DNA polymerase, 20 ng template DNA, 0.2 mmol·L^-1 dNTPs, 0.3 μmol·L^-1 primer and 2.5 mmol·L^-1 Mg²⁺. This optimum reaction system was applied to fingerprint 8 varieties of R.tomentosa accessions by Me1-Em11 primers and produced clear polymorphic patterns. It showed that the optimized system could be effectively applied in the germplasm identification and genetic diversity analysis of R.tomentosa.

Key words: Rhodomyrtus tomentosa(Ait.) Hassk., uniform design, SRAP, system optimization