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Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (2): 146-151.doi: 10.7525/j.issn.1673-5102.2010.02.004

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Characteristic of Molecular Evolution of Sibiraea angustata Based on nrDNA ITS and cpDNA trnL-F Sequence Analysis

DUAN Yi-Zhong;ZHANG De-Jun;GAO Qing-Bo;ZHANG Fa-Qi;LI Yin-Hu;CHEN Shi-Long*   

  1. 1.Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001;2.Graduate University of Chinese Academy of Sciences,Beijing 100049
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: CHEN Shi-Long
  • Supported by:

Abstract: The differences between the nrDNA ITS and cpDNA trnL-F sequences in Sibiraea angustata were analyzed by PCR direct sequencing, and then compared with cpDNA trnS-G, rpl20-rps12 sequences of this species. Total DNA was extracted from silica-dried leaves of S.angustata using modified CTAB method. With the extracted DNA as template, nrDNA ITS and cpDNA trnL-F regions were amplified, then purified and sequenced. The length of nrDNA ITS sequence of S.angustata was 601 bp, of which 3 were variable sites with a percentage of 0.05%, the (G+C) content was 41.4%. The length of cpDNA trnL-F sequence of S.angustata was 927 bp, of which only one was variable site with a percentage of 0.01%, the(G+C) content was 32.6%. The nrDNA ITS region of S.angustata was more conserved and evolved more slowly than the cpDNA trnS-G and rpl20-rps12 sequences, but evolved a little more rapidly when compared to cpDNA trnL-F. The present distribution range of S.angustata has experienced range expansion by the haplotype analysis of this species,which consists with the conclusion resulting from cpDNA genome. Therefore, the nrDNA ITS sequence of S.angustata was fit to the phylogeographic study of this species.

Key words: Sibiraea angustata, ITS sequence, trnL-F sequence, trnS-G sequence, rpl20-rps12 sequence

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