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Bulletin of Botanical Research ›› 2007, Vol. 27 ›› Issue (3): 297-301.doi: 10.7525/j.issn.1673-5102.2007.03.010

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Comparison of the Protein Composition and Analysis of the Specific Protein from Wheat-Haynaldid villosa Chromosome Substitution Line and Translocation Line by 2D-PAGE

FAN Bao-Li;LI Chun-Zheng;PENG Yong-Kang*   

  1. Department of Biology,Tianjin Normal University,Tianjin　300074
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-20 Published:2007-05-20
  • Contact: PENG Yong-Kang
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Abstract: With 2D-PAGE techniques, comparison of leaf protein in cultivar wheat (AABBDD), Wheat-Haynaldia villosa chromosome substitution line (6V/6A), translocation lines (6VS/6AL), (6VS/6DL) and H. villosa (VV) have been made in this study. Over 350 protein species were detected in the leaf of cultivar wheat, chromosome substitution line and two translocation lines. Their molecular weight ranged from 10~110 KD and pI from 4.5～8.6. 2D-PAGE patterns of leaf protein are almost the same among cultivar wheat, 6V/6A, 6VS/6AL, 6VS/6DL; but different from H. villosa. A specific protein (16 KD/pI5.0) was found in substitution line, two translocation lines and H.villosa, but not found in the cultivar wheat. These results suggested that the protein (16 KD/pI5.0) may be located in the short arm of V chromosome of H. villosa.

Key words: chromosome substitution line, 2D-PAGE, Haynaldia villosa, powdery mildew, specific protein, translocation lines

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