Abstract: 25 species and 4 varieties are reported here as new records in Guizhou Province，P. R. China. They are Acer heptalobum Diels., A. longipes Franch ex Rehd., Rhododendron fuyuanense Z. H. Yang., R. delavayi Franch. var. pilostylum. K. M. Feng., Gaultheria wardii Marq. & Airy Shaw., Nomocharis forrestii Balf., Polygonatum sibircum Delar. ex Redouté., P. verticillatum (Linn.) All., P. gracile P. Y. Li.，Disporum calcaratum D. Don., Tricyrtis latifolia Maxin., Ranuculus diffuses DC.，R. trigonus Hand.—Mazz.，R. kunmingensis W. T. Wang., R. kunningensis W. T. Wang var. hispidus W. T. Wang., Rubus aurantiacus Focke., R. xichouensis Yü & Lu., R. subornatus Fooke var. melanadenus Focke., R. phoenicolasius Maxim.，R. pentagonus Wall. ex Focke.，Neillia thibetica Bur. & Franch. var. lobata (Rehd.) Yü.，N. affinis Hemsl., Crataegus maximowiczii Schneid.，Berberis weixiensis C. Y. Wu ex S. Y. Bao., B. fallaciosa Schneid，B. metopolyantha Ahrendt., Mahonia napaulensis DC., M. longibracteata Takeda. and Boschniakia himalaica (Hook. f. & Thoms.) G. Beck. The habitats and geographical distribution of these taxa are presented in this Paper.

Key words: Spermatophyte, new records, geographical distribution, Guizhou Province