Abstract: Proteins in under-ground stem,stem and leaf tissues of Polygonum sibiricum which were watered with 3% NaHCO 3 for 5 days,10 days and with tap water for control were extracted by phenol extraction method and separated and analyzed by SDS-PAGE and the related software labword version 3.0.The results show that two bands were up-regulated in the map of the under-ground tissue with the molecular weights of 34.1×10³D and 25.0×10³D,three bands were up-regulated in the map of the stem tissue with the molecular weights of 58.1×10³D、47.8×10³Dand 9.6×10³D and one band was up-regulated in the map of the leaf tissue with the molecular weight of 25.0×10³D.Apart from the same band at 25×10³D position on the maps of the under-ground stem tissue and the leaf tissue,the bands at other positions on the maps of three parts of the plant which were up-regulated were different.The diversities of the patterns of protein expression in different parts of the plant are illuminated by the results that were gained.

Key words: Polygonum sibiricum, salt-stress, phenol extraction method, SDS-PAGE