Abstract: The effects of culture environment on callus growth and content of dl-tetrahydro-palmatine (THP) in the cultures from stem tubers of Corydalis remota were discussed. The results showed that 2, 4-D was better for growth of callus cells than NAA. The dry weight of the callus and level of THP were promoted when 2 mg/L 2, 4-D and 1 mg/L BA were added to the medium. Sucrose increased the fresh weight of callus, and the combination of sucrose and galactose promoted the increase of callus dry weight and raise of THP level, significantly. Either casein hydrolysate (CH) or lactolbumin hydrolysate (LH) was favored to the growth of callus and raise of THP level, and there was highest level of THP in the group treated with 450 mg/L LH. Light enhanced the growth of callus and raise the level of THP.

Key words: Corydalis remota, callus culture, THP, culture environment