Abstract: The protoplasts of three intergeneric grasses (Psathyrostachys juncea, Bromus inermis and Bromus willdenowii Kunth) were fused with wheat (Triticum aestivum. Jinan 177) protoplasts by PEG method respectively. The fusion products of wheat and grasses were analyzed with morphological inspection, isozyme analysis, 5S rDNA spacer sequence analysis. Parts of hybrid clones were taken for chromosome examination. Different chromosome compositions in calli were found between the fusion combinations of different phylogenetical distance, while microchromosomes or chromosome fragments were always present. The reason for this result and the failure of plant regeneration in this experiment is discussed.

Key words: Triticum aestivum, Intergeneric grasses, Symmetric somatic hybridization, Hybrid chromosome composition