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Bulletin of Botanical Research ›› 1997, Vol. 17 ›› Issue (1): 89-95.

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STUDIES ON FERTILITY RESTORER GENE Rf3 AGAINST G-TYPE CYTOPLASMIC MALE STERILE IN WHEAT Ⅱ GENOMIC LIBRARY CONSTRUCTION OF T.TIMOPHEEVI USING TOTAL DNA

Gao Dong-jie, Cao Hai-ming, Lin Jiang-nan, Wang Tong-chang   

  1. Department of Biology, Harbin Normal University, Harbin 150080
  • Received:1996-09-01 Online:1997-03-15 Published:2016-06-14

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Abstract: Genomic DNA of T.timopheevi was digested with three restriction enzymes, Pst Ⅰ, Hind Ⅲ and EcoR I.The fragments were ligated with the plasmid pUC119 which was treated with the some enzyme before.The transformation efficiency is 1.20×104/μg.Recombinants were determined by means of α-complementary test, agarose gel electrophoresis and dot hybridization.The recombination frequency is 43.8%.

Key words: Fertility restorer, Genomic library, Dot hybridization, Triticum timopheevi