Analysis of Growth Traits and Anatomical Structures of Doubled Haploid Lines of Populus simonii × P. nigra

doi:10.7525/j.issn.1673-5102.2025.06.006

Abstract

Abstract:

Compared with traditional breeding method， the doubled haploid （DH） induction technology can effectively shorten the breeding cycle and quickly obtain homozygous lines of trees. Due to the difficulty in inducing DH in trees， it is hard to establish large-scale DH genetic populations， therefore， there are currently few reports on the genetic variation of DH populations in trees. In this study， the growth traits， leaf phenotypes and anatomical characteristics of donor tree of Populus simonii × P. nigra and four DH lines were analyzed. The results showed that the DH lines had obvious growth defects. The height， ground diameter， number of stem nodes， leaf length， leaf width and leaf area of DH lines were significantly lower than those of donor trees. Anatomical analysis of the apical buds revealed distinct morphological differences in the apical meristem growth points between donor trees and DH lines. The observation and analysis of the paraffin sections of the stems and the tissue staining showed that the vascular cambium of DH lines was relatively smaller， the average area of each vessel cell was smaller， and the number of vessel and fiber cells was significantly higher than that of donor trees. This study revealed the defects of DH lines in secondary growth， and will provide a material basis for subsequent research on the function analysis of specific genes and the optimization of breeding strategies.

Key words: Populus simonii × P. nigra, doubled haploid, growth traits, anatomical structures

CLC Number:

S722.3

Yunbo XU, Heng ZHANG, Caixia LIU, Meng WANG, Yuhang LIU, Qiuyu WANG, Mingyuan MAI, Guanzheng QU. Analysis of Growth Traits and Anatomical Structures of Doubled Haploid Lines of Populus simonii × P. nigra[J]. Bulletin of Botanical Research, 2025, 45(6): 888-897.

Figures/Tables 9

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亲本及双单倍体无性系 DT and DH lines	第5片叶 The fifth leaf			第6片叶 The sixth leaf			第7片叶 The seventh leaf			第8片叶 The eighth leaf
亲本及双单倍体无性系 DT and DH lines	叶长 Leaf length /cm	叶宽 Leaf width /cm	叶长宽比 Leaf aspect ratio	叶长 Leaf length /cm	叶宽 Leaf width /cm	叶长宽比 Leaf aspect ratio	叶长 Leaf length /cm	叶宽 Leaf width /cm	叶长宽比 Leaf aspect ratio	叶长 Leaf length /cm	叶宽 Leaf width /cm	叶长宽比 Leaf aspect ratio
DT	7.03±1.93^a	6.33±2.72^a	1.19±0.27^b	7.27±1.88^a	7.20±2.43^a	1.03±0.11^b	8.43±0.32^a	8.30±0.36^a	1.02±0.04^d	9.13±0.35^a	8.47±0.23^a	1.08±0.02^c
DH1588	2.67±0.15^bc	2.47±0.58^b	1.12±0.24^b	3.20±0.10^b	3.30±0.17^bc	0.97±0.08^b	2.87±0.67^c	2.90±0.52^bc	0.98±0.06^d	2.90±0.26^c	2.50±0.10^c	1.16±0.07^bc
DH3-50	4.50±0.61^b	3.57±0.49^b	1.26±0.03^ab	4.20±0.70^b	3.87±0.49^b	1.08±0.06^b	4.37±0.58^b	3.53±0.55^b	1.24±0.04^c	4.83±0.55^b	3.97±0.12^b	1.22±0.13^bc
DH1717	3.63±0.81^bc	2.53±0.61^b	1.44±0.03^ab	3.40±0.44^b	2.07±0.21^bc	1.66±0.30^a	3.73±0.47^bc	2.60±0.35^c	1.44±0.04^b	3.63±0.40^c	2.70±0.26^c	1.34±0.03^b
DH1716	2.23±0.32^c	1.43±0.23^b	1.56±0.05^a	2.37±0.15^b	1.50±0.30^c	1.61±0.22^a	3.53±0.15^bc	2.17±0.31^c	1.65±0.18^a	3.73±0.47^c	2.33±0.45^c	1.62±0.20^a