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Bulletin of Botanical Research ›› 2005, Vol. 25 ›› Issue (4): 415-418.doi: 10.7525/j.issn.1673-5102.2005.04.008

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Expression of Metallothionein-II in Arabidopsis thaliana improve desiccation tolerance

TANG Zhong-Hua, GUO Xiao-Rui, ZHANG Yang-Yang, AN Zhi-Gang, YU Jing-Hua, ZU Yuan-Gang   

  1. Key Laboratory of Forest Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:2005-07-28 Online:2005-12-15 Published:2016-06-14
  • Supported by:
    Supported by the State Key Basic Research Project (G19990160) and the Key Project of Chinese Ministry of Education (104191)

Abstract: Metallothionein-II(MT-II) has been identified to play an important role in the exposure of plant to heavy metal like Cd and Cu.One of the mechanisms of MT's proposed to protect plants during this process is that it can quench the reactive oxygen species(ROS) activated by heavy-metal exposure.Here we demonstrated the effect of expression of MT-II in Arabidopsis thaliana on oxidative stress and damage extent during drought stress. The RT-PCR results suggested the implication of MT-II against drought stress in the transgenic Arabidopsis.Measurements of H2O2 and malondialdehyde (MDA) indicated that over-expression of MT-II in A.thaliana improved desiccation tolerance.

Key words: MT-II, desiccation tolerance, scavenging ROS