关键词: RAPD, 混合分组分析, 棱果沙棘

Abstract: This article used the methods of random amplified polymorphic DNA(RAPD) with bulked segregant analysis(BSA) to identify markers that linked to sex determination in the dioecious Hippophae goniocarpa. DNA was extracted and two bulks were created, each containing equal amounts of DNA from ten individual of Hippophae goniocarpa. A total of 194 primers were screened. 50 primers produced clear patterns with at least one band that appeared to be polymorphic between male and female bulks. Sebsequently, 5 male and female individuals were analysed with those 50 primers, and only S10(CTGCTGGGAC) could generate a common 1030 bp DNA fragment in all five female individuals and female pool but not in any male individuals. In the present study, the sex-linked RAPD marker, S10-1030, showed that there were different between female and male Hippophae goniocarpa on the level of DNA molecule and can be concluded that gender of Hippophae goniocarpa most likely is genetically determined. While the further research should focus on designing the more reliable molecular marker that based on the sex-determination gene, such as SCAR marker, for accurate and quick determination the gender of seedling in Hippophae goniocarpa.

Key words: RAPD, BSA, Hippophae goniocarpa