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植物研究 ›› 2013, Vol. 33 ›› Issue (2): 191-196.doi: 10.7525/j.issn.1673-5102.2013.02.012

• 论文 • 上一篇    下一篇

基因枪法获得无选择标记的1Dx5基因表达的转基因小麦

王勇1;覃建兵1;范玲2;祝长青1*   

  1. 1.新疆师范大学分子生物学与生物信息研究室,乌鲁木齐 830053;2.新疆农业科学院,乌鲁木齐 830091
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-03-20 发布日期:2013-03-20
  • 通讯作者: 祝长青
  • 基金资助:
     

Transformation and Expression of 1Dx5 without Selectable Markers in Common Wheat Xindong No.26

WANG Yong;QIN Jian-Bing;FAN Ling;ZHU Chang-Qing*   

  1. 1.Research Room of Molecular Biology and Informatics,Xinjiang Normal University,Urumqi 830053;2.Xinjiang Academy of Agricultural Sciences,Urumqi 830091
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-03-20 Published:2013-03-20
  • Contact: ZHU Chang-Qing
  • Supported by:
     

摘要: 利用基因枪将无选择标记的优质高分子量麦谷蛋白亚基基因1Dx5导入新疆耐盐小麦品种新冬26,为利用优质基因进行小麦品质改良奠定基础。构建无选择标记的线性1Dx5表达框。利用基因枪将其转入不含该亚基的小麦品种新冬26幼胚盾片中,经PCR二分法筛选,从转化的1 000块幼胚盾片中共获得3株转基因阳性植株,转化效率0.3%。利用SDS-PAGE分析目的基因在转基因后代籽粒中的表达。转基因植株后代种子分析表明,1Dx5在转基因后代部分种子中表达。本研究成功地将无选择标记的线性1Dx5片段导入普通小麦新冬26中,并在后代部分种子中得到了表达。为利用优质亚基基因改良小麦加工品质奠定基础。

关键词: 小麦, 选择标记, 1Dx5, 基因枪

Abstract: To improve the bread-making quality of wheat, the Xinjiang winter common wheat Xindong No.26 were transformed with high-molecular-weight glutenin subunits (HWM-GS) gene 1Dx5 that without selectable markers by particle bombardment. Transformed plants were screened by PCR, three transgenic plants were detected among 1 000 transformed plants, yielding a transformation efficiency of 0.3%. The compositions of HWM-GS were analyzed by SDS-. The HMW-GS gene 1Dx5 was expressed in some seeds of transgenic plants. The high molecular weight glutenin subunit gene 1Dx5 was transformed into wheat XinDong No.26 and was expressed in some transgenic progenies. Our studies lay the foundations for the improvement of bread-making quality of wheat.

Key words: wheat, selectable markers, 1Dx5, microprojectile

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