He-Ne激光和增强UV-B辐射对小麦幼苗类囊体捕光色素的影响

doi:10.7525/j.issn.1673-5102.2010.01.008

植物研究 ›› 2010, Vol. 30 ›› Issue (1): 32-36.doi: 10.7525/j.issn.1673-5102.2010.01.008

He-Ne激光和增强UV-B辐射对小麦幼苗类囊体捕光色素的影响

高丽美;李永锋;韩榕^*

山西师范大学生命科学学院，临汾　041004

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-20 发布日期:2010-01-20
通讯作者: 韩榕
基金资助:

Effects of He-Ne Laser and Enhanced UV-B Radiation on Chloroplast Pigments in Wheat Seedlings

GAO Li-Mei;LI Yong-Feng;HAN Rong*

College of Life Science,Shanxi Normal University,Linfen　041004

Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
Contact: HAN Rong
Supported by:

摘要/Abstract

摘要： 采用5 mW·mm^-2 He-Ne激光辐照、10.08 kJ·m^-2d^-1 UV-B辐射及二者组合对冬小麦幼苗进行处理。通过测定叶绿体捕光色素含量和色素蛋白组成的变化，进一步探讨He-Ne激光对增强UV-B辐射后小麦幼苗类囊体捕光色素损伤的修复效应。循环处理小麦幼苗4 d，利用90%乙醇和80%丙酮分别提取各处理组小麦幼苗叶片中的叶绿素,通过纸层析和分光光度法检测捕光色素含量的变化，并探讨不同处理对叶绿素与蛋白质结合牢固性的影响。利用柱层析法测定色素蛋白的主要成分。研究表明：与对照组相比，增强UV-B辐照后小麦幼苗捕光色素总含量降低了17.76%，叶绿素和蛋白质结合牢固度显著降低,色素蛋白的组成也发生变化，D1和D2蛋白质条带消失；而一定剂量He-Ne激光辐照可使增强UV-B辐射后的叶绿体色素含量增加约10.64%，但仍低于ck组约8.12%,叶绿素和蛋白质结合牢固度也显著高于B组，色素蛋白的组成与对照组相似。因此，低剂量的He-Ne激光辐照对增强UV-B辐射后小麦幼苗类囊体捕光色素的损伤具有促进修复效应。

关键词: He-Ne激光, UV-B辐射, 捕光色素, 小麦

Abstract: The seedlings of wheat were exposed to He-Ne laser(5 mW·mm^-2) irradiation and enhanced UV-B(10.08 kJ·m^-2d^-1) radiation. Then the content changes of chloroplast pigments and the composition of pigment-proteins were measured in order to test the mitigative effect of He-Ne laser irradiation on injury by enhanced UV-B radiation. After treating wheat seedlings for 4 days circularly, chloroplast pigments were extracted respectively by 90% ethanol and 80% acetone. The contents of chloroplast pigments were detected with spectrophotometer or paper chromatography. The composition of pigmentproteins were detected with column chromatography. It suggested that the content of chloroplast pigments decreased 17.76% approximately in wheat seedlings by enhanced UV-B radiation. The binding degree of Chl-Pro is weakened. The composition of pigment-proteins also differed from the control group. Straps of D1 and D2 proteins lost. However, the content of chloroplast pigments increased about 10.64% exposed to He-Ne laser irradiation, but decreased 8.12% comparing with the control. The binding degree of Chl-Pro is higher than the B group. At the same time, the composition of pigmentproteins was similar with the control group. So the lower dose of He-Ne laser irradiation can partly repair the radiation injury on chloroplast pigments and pigment-proteins in wheat seedlings after enhanced UV-B radiation.

Key words: He-Ne laser, ultraviolet-B radiation, chloroplast pigment, wheat

中图分类号:

Q256

高丽美;李永锋;韩榕*. He-Ne激光和增强UV-B辐射对小麦幼苗类囊体捕光色素的影响[J]. 植物研究, 2010, 30(1): 32-36.

GAO Li-Mei;LI Yong-Feng;HAN Rong*. Effects of He-Ne Laser and Enhanced UV-B Radiation on Chloroplast Pigments in Wheat Seedlings[J]. Bulletin of Botanical Research, 2010, 30(1): 32-36.

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He-Ne激光和增强UV-B辐射对小麦幼苗类囊体捕光色素的影响

Effects of He-Ne Laser and Enhanced UV-B Radiation on Chloroplast Pigments in Wheat Seedlings

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