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植物研究 ›› 1990, Vol. 10 ›› Issue (4): 113-119.

• 论文 • 上一篇    下一篇

小黑杨花粉植株过氧化物酶同工酶分析

刘玉喜, 陆志华, 詹亚光, 李莉, 齐立志, 张培果   

  1. 东北林业大学, 黑龙江哈尔滨
  • 出版日期:1990-12-15 发布日期:2016-06-14

PEROXIDE ISOZYME ANALYSIS IN POLLEN PLANTS OF POPULUS XIAOHEI

Liu Yu-xi, Lu Zhi-hua, Zhan Ya-guang, Li Li, Qi Li-zhi, Chang Pei-gao   

  1. Northeast Forestry Univergity Harbin Heilongjiang
  • Online:1990-12-15 Published:2016-06-14

摘要: 1989年冬笔者对已自行加倍的80株小黑杨花粉植株的休眠芽进行了过氧化物酶同工酶分析,发现株间有差异,酶谱可截然分成两类。第一类酶谱与小黑杨原株(花药培养供体)不同,在B带区多了一条带纹即B2带,此类约占78%。第二类酶谱与小黑杨原株完全相同,此类约占22%。无论株间形态分离还是过氧化物酶同工酶酶谱差异都充分说明这些小黑杨单倍体植株来源于花粉,它们是重组花粉在植株水平上的表现。实验还证明,小黑杨花粉植株休眠芽的过氧化物酶同工酶酶谱是稳定的,可用来鉴定小黑杨花粉植株的无性系,相信用同工酶鉴定杨树无性系会对林业生产起重要作用。

Abstract: The writers analysed dormant buds of 80 self-redoubled pollen plants of P. xiaohei for peroxide isozyme zymograms in winter 1988, and found difference between the plants. The zymograms of them could be sharply devided into two types. One was complete same as that of original plant and made up 22%, the other was different, and there was a Bband more than the zymogram of original plant in B region, and this type amounted to 78%. Whether the shape segregation among plants or the difference of peroxide isozyme zymograms, it was fully showed that the haploid plants of P. xiaohei originated from pollen. They were the expression of pollens recombinated in chromosomes at plant lever, the experiment also proved that the peroxide isozyme zymograms of dormand buds from pollen plants of P. xiaohei were stable and were able to be used for identifing poplar clones. Therefore, using peroxide isozyme to identify clones of poplar would give important role in forestry production.