火炬松胚性悬浮细胞原生质体的分离和培养

植物研究 ›› 1997, Vol. 17 ›› Issue (3): 320-324.

火炬松胚性悬浮细胞原生质体的分离和培养

唐巍¹, 欧阳藩¹, 郭仲琛²

1. 中国科学院化工冶金研究所, 北京 100080;
2. 中国科学院植物研究所, 北京 100093

收稿日期:1997-01-01 出版日期:1997-09-15 发布日期:2016-06-14
基金资助:
国家"863"资助课题

ISOLATION AND CULTURE OF PROTOPLAST FROM EMBRYONIC SUSPENSION CELL OF PINUS TAEDA LINN.

Tang Wei¹, Ou Yang-Fan¹, Guo Zhong-chen²

1. Institute of Chemical Metallurgy, Academia Sinica, Beijing, 100080;
2. Institute of Botany, Academia Sinica, Beijing, 100093

Received:1997-01-01 Online:1997-09-15 Published:2016-06-14

摘要/Abstract

摘要： 以火炬松(Pinus taeda L.)成熟合子胚的胚性悬浮细胞为材料分离原生质体,研究了酶液组成、渗透压稳定剂和悬浮细胞生长期对原生质体产量和原生质体活力的影响。结果表明:酶液组成为Cellulase RS 1%+Cellulase R-102.5%+Pectolyase Y-230.2%、渗透压稳定剂为甘露醇,在悬浮细胞处于对数生长期时,原生质体的产量和活力均最高。高活力的原生质体在DC R培养基上培养6d后完成第1次分裂,4周后形成胚性细胞团,8周后形成胚性胚柄细胞团(ESM:Embryonal suspensor mass),16周后胚性顶端分化出子叶原基。

关键词: 火炬松, 原生质体, 胚性胚柄细胞团

Abstract: The influences of enzyme combination,osmoticum,and suspension cell growth phase on both protoplast yield and protoplast viability were investigated with embryonal suspension cell from mature zygotic as materials of isolation protoplast.The results show that protoplast yield and protoplast viability of suspension cells on linear phase are the highest under the enzyme combination cellulase RS 1%+cellulase R-10 2.5%+pectolyase Y-23 0.2% and osmoticum mannital.After protoplast were cultured on DCR medium,the first division of protoplast appeared on 6th day,embryonic cell mass appeared on 4th week,embryonal suspensor mass appeared on 8th week,and somatic embryo with primary cotyledonary appeared on 16th week.

Key words: Pinus taeda L., Protoplast, Embryonal suspensor mass

唐巍, 欧阳藩, 郭仲琛. 火炬松胚性悬浮细胞原生质体的分离和培养[J]. 植物研究, 1997, 17(3): 320-324.

Tang Wei, Ou Yang-Fan, Guo Zhong-chen. ISOLATION AND CULTURE OF PROTOPLAST FROM EMBRYONIC SUSPENSION CELL OF PINUS TAEDA LINN.[J]. Bulletin of Botanical Research, 1997, 17(3): 320-324.

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