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植物研究 ›› 2006, Vol. 26 ›› Issue (1): 70-73.doi: 10.7525/j.issn.1673-5102.2006.01.016

• 论文 • 上一篇    下一篇

普通针毛蕨配子体发育的研究

张开梅1,2;石 雷1*;李 东1   

  1. 1.中国科学院植物研究所,北京 100093 2.中国科学院研究生院,北京 100039
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-01-20 发布日期:2006-01-20
  • 通讯作者: 石 雷
  • 基金资助:
     

Observation on the gametophyte development of Macrothelypteris torresiana

ZHANG Kai-Mei;SHI Lei*;LI Dong   

  1. 1.Institute of Botany, the Chinese Academy of Sciences, Beijng 100093 2.Graduate School of the Chinese Academy of Sciences, Beijing 100039
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-01-20 Published:2006-01-20
  • Contact: SHI Lei
  • Supported by:
     

摘要: 采用混合土培养普通针毛蕨(Macrothelypteris torresiana (Gaud.) Ching)孢子,显微镜下观察记录其孢子萌发及配子体发育过程。结果表明:孢子褐色,赤道面观为半圆形,极面观椭圆形,单裂缝。播种3 d左右孢子萌发,萌发类型为书带蕨型,配子体发育为槲蕨型。播种7 d左右发育为片状体,丝状体与片状体两阶段的界限不明显。原叶体边缘及背腹面均可产生毛状体。播种21 d左右发育形成幼原叶体,成熟原叶体呈心脏形。播种35 d左右开始有性器官出现,精子器近圆球形,由3个细胞组成,成熟颈卵器颈部由4列细胞组成,4~5层细胞高。原叶体受精后1个月内即可观察到从原叶体上生成的幼胚。

关键词: 普通针毛蕨, 孢子萌发, 配子体发育

Abstract: The spores of the Macrothelypteris torresiana (Gaud.) Ching were cultured in the mixed soil. The spore germination and the gametophytic development were observed by microscope. The result exhibits that the spores are brown, monolete, and semicircle-shaped in equatorial view and elliptical in polar view. The spores germinate about three days after sowing. The germination is of the Vittaria-type while the gametophyte development is of Drynaria-type. The prothallial plates come into form about seven days after sowing. The radius is not so obvious between the germ filaments stage and the prothallial plates stage. Hairs can occur on both the surfaces and margin of the prothalli. The young prothalli develop in 21 days after sowing and the mature prothalli are cordate. The Antheridium form in 35 days after sowing. The antheridia are like pellets and composed of 3 cells. The archegonial neck is usually formed by 4 rows of cells and 4 to 5 cells high at maturity. Young embryos can be observed growing from the prothalli in one month after sowing.

Key words: Macrothelypteris torresiana (Gaud.) Ching, spore germination, gametophyte development

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