培养条件对齿瓣延胡索愈伤组织生长及延胡索乙素含量的影响

植物研究 ›› 2003, Vol. 23 ›› Issue (1): 86-90.

培养条件对齿瓣延胡索愈伤组织生长及延胡索乙素含量的影响

张东向

齐齐哈尔大学生命科学与工程学院, 齐齐哈尔 161006

收稿日期:2001-03-16 出版日期:2003-03-15 发布日期:2016-06-14
作者简介:张东向(1963-),男,理学硕士,副教授,从事植物生理学与植物细胞工程研究。
基金资助:
齐齐哈尔市青年基金项目

EFFECTS OF CULTURE ENVIRONMENT ON CALLUS GROWTH AND DL-TETRAHYDRO-PALMATINE CONTENT IN CULTURES OF CORYDALIS REMOTA

ZHANG Dong-Xiang

College of Life Science and Technology, Qiqihaer University, Qiqihaer 161006

Received:2001-03-16 Online:2003-03-15 Published:2016-06-14

摘要/Abstract

摘要： 研究了不同培养条件对齿瓣延胡索(Corydalis remota)愈伤组织生长及延胡索乙素(THP)含量的影响。结果表明, 2, 4-D较NAA更有利于提高培养物鲜重,加速细胞增殖。培养基添加2.0 mg/L 2, 4-D和1.0 mg/L BA不仅有利于干物质积累,而且显著提高THP水平。蔗糖不仅可提高培养物鲜重,而且与半乳糖组合可显著促进干物质积累和THP水平的提高。水解酪蛋白和水解乳蛋白均有利于愈伤组织生长和THP含量的提高, 450mg/L的水解乳蛋白处理组THP含量最高。光照可提高培养物鲜重和THP含量。

关键词: 齿瓣延胡索, 愈伤组织培养, 延胡索乙素, 培养条件

Abstract: The effects of culture environment on callus growth and content of dl-tetrahydro-palmatine (THP) in the cultures from stem tubers of Corydalis remota were discussed. The results showed that 2, 4-D was better for growth of callus cells than NAA. The dry weight of the callus and level of THP were promoted when 2 mg/L 2, 4-D and 1 mg/L BA were added to the medium. Sucrose increased the fresh weight of callus, and the combination of sucrose and galactose promoted the increase of callus dry weight and raise of THP level, significantly. Either casein hydrolysate (CH) or lactolbumin hydrolysate (LH) was favored to the growth of callus and raise of THP level, and there was highest level of THP in the group treated with 450 mg/L LH. Light enhanced the growth of callus and raise the level of THP.

Key words: Corydalis remota, callus culture, THP, culture environment

张东向. 培养条件对齿瓣延胡索愈伤组织生长及延胡索乙素含量的影响[J]. 植物研究, 2003, 23(1): 86-90.

ZHANG Dong-Xiang. EFFECTS OF CULTURE ENVIRONMENT ON CALLUS GROWTH AND DL-TETRAHYDRO-PALMATINE CONTENT IN CULTURES OF CORYDALIS REMOTA[J]. Bulletin of Botanical Research, 2003, 23(1): 86-90.

[1]	张玉红;曲伟娣. 培养条件对黄檗快速繁殖影响的研究[J]. 植物研究, 2008, 28(2): 236-239.
[2]	张东向, 李显峰, 邵淑丽. 东北延胡索愈伤组织培养及延胡索乙素形成的适宜条件[J]. 植物研究, 2003, 23(3): 302-307.