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植物研究 ›› 2004, Vol. 24 ›› Issue (1): 101-106.

• 论文 • 上一篇    下一篇

菜豆种质形态及等位酶标记的研究

段喜华1, 栾非时2, 孙晶波3   

  1. 1. 东北林业大学, 哈尔滨 150040;
    2. 东北农业大学园艺学院, 哈尔滨 150030;
    3. 黑龙江省林业厅, 哈尔滨 150030
  • 收稿日期:2003-01-04 出版日期:2004-03-15 发布日期:2016-06-14
  • 作者简介:段喜华(1961-), 女, 副教授, 主要从事植物生态学研究。

The morphology and allozyme of germplasm resources on Phaseolus vulgaris

DUAN Xi-Hua1, LUAN Fei-Shi2, SUN Jing-Bo3   

  1. 1. Northeast Foreast University, Harbin 150040;
    2. Northeast Agricultural University, Harbin 150030;
    3. Forestry Bureau of Heilongjiang, Harbin 150030
  • Received:2003-01-04 Online:2004-03-15 Published:2016-06-14

摘要: 收集了黑龙江省43 份栽培品种, 国际热带农业中心13 份半野生品种, 波兰4 份矮生品种共60 份菜豆品种资源, 测定其植物学、生物学性状, 采用标准差标准化方法, 计算各材料间的欧氏距离, 其范围为0.110~0.985;从等位酶标记上进行了10 个酶系统检测, 共获得25 个位点, 多态位点比率为0.16, 其平均遗传距离为0.056 8 。根据计算所得60 份材料间的遗传相似度, 开展形态标记亲缘关系系统聚类图, 将其分为两大类, 即矮生种为一类, 蔓生种及半野生种合为一类。采用平均距离法, 将等位酶标记聚成8 大类, 其中蔓生种占5 类, 矮生种2 类, 半野生种2 类, 说明等位酶标记较具体, 且蔓生种遗传距离较远, 可作为育种材料储备种质资源。

关键词: 菜豆, 种质, 标记, 等位酶

Abstract: Sixty varieties of Phaseolus vulgaris were collected in this study, including 43 from Heilongjiang Province, 13 from The Center of International Agriculture Tropic and 4 from Poland, The botanical and biological characters of the 60 Phaseolus vulgaris varieties were measured.The range of the Oushi's distance among the varieties was 0.11~0.985, calculated by the normalization of standard derivation and our own computer program.The analysis on 10 enzyme systems was done and 25 locaties were obtained.The ratio of polymorphic loci was 0.16.The average genetic distance was 0.056 8.Based on the analysis of genetic relationship of the dendrogram, the 60 Phaseolus vulgaris varieties could be classified into two types, one for dwarf variety, and one for the vine and semi-wild varieties.By using the average genetic distance of Allozyme, the 60 varieties could be divided into 8 groups, 5 for vine, 2 for dwarf, and 2 for semi-wild variety. The genetic distance of vine variety was relatively far, suggesting its importance for storing germplasm resource as breeding materials.

Key words: Phaseolus vulgaris, germ plasm, morphology, allozyme