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植物研究 ›› 2012, Vol. 32 ›› Issue (5): 567-572.doi: 10.7525/j.issn.1673-5102.2012.05.011

• 论文 • 上一篇    下一篇

干旱胁迫与不同储藏时间对杠柳种子萌发的影响

李鹏鹏;张文辉*   

  1. 天津师范大学生命科学学院,天津 300387
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-09-20 发布日期:2012-09-20
  • 通讯作者: 张文辉
  • 基金资助:
     

Seed Germination of Periploca sepium under Drought Stress and Different Storage Time

LI Peng-Peng;ZHANG Wen-Hui*   

  1. College of Life Sciences,Tianjin Normal University,Tianjin 300387
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-09-20 Published:2012-09-20
  • Contact: ZHANG Wen-Hui
  • Supported by:
     

摘要: 为了阐明干旱胁迫与不同储藏时间对杠柳种子萌发的影响机理,在室内采用不同浓度的聚乙二醇(PEG)模拟干旱胁迫条件,对3种不同储藏时间的杠柳种子进行了萌发期对比试验,观测了种子的总萌发率、发芽势、活力指数和胚根的生长情况,分析了抗旱特性,结果表明:不同程度的干旱胁迫与储藏时间对杠柳种子萌发均有一定的影响,当PEG-6000浓度为5%,对储藏1年杠柳种子的萌芽率和发芽势起促进作用,对储藏2年种子萌芽率轻度抑制,对储藏3年种子抑制影响较大,当PEG-6000浓度>5%,3种种子的萌芽率和发芽势均呈下降趋势,但当胁迫浓度增大到25%时,种子基本没有萌发。在不同的水分胁迫处理中,3种种子的活力指数和胚根生长速度一直呈下降趋势,尤其3年种子的下降幅度最大。不同储藏时间杠柳种子对干旱胁迫的适应性有差异,储藏1~2年的杠柳种子在PEG-6000浓度为5%~15%中萌发效果较好,且储藏1年种子好于2年种子,3年种子较差。3种种子的耐旱能力由强到弱依次为:1年种子>2年种子>3年种子。

关键词: 杠柳, 总萌发率, 发芽势, 活力指数, 胚根生长

Abstract: In order to clarify the mechanism of Periploca Sepium seed germination under drought stress and different storage time, different concentrations of polyethylene glycol (PEG) were used to simulate drought stress condition and germination period of comparison tests were carried out on three different storage time seeds. The total germination rate, germination force, vitality index and radicle growth were observed and the drought resistance was analyzed. The results showed that: different levels of drought stress and storage time had a certain influence on seed germination. Five percentages of PEG-6000 could promote the one-year seeds germination rate and germination force, mildly suppressed two-year seeds, greater influenced three-year seeds. When PEG-6000 concentration was higher than 5%, the germination rates and germination forces of three kinds of seeds decreased, while little seed germinated when the stress concentration increased to 25%. The vitality index and radicle growths of three kinds of seeds decreased, in particular in different water stress, the biggest drop was three-year seed. The best germination condition was storage for one to two years seeds in PEG-6000 5%-15%, and seeds storage for 1 year better than 2 years seeds, 3 years seeds was the worst. The drought-tolerance of three kinds of seeds decreased in the following order: storage 1 year seeds>2 years seeds>3 years seeds.

Key words: Periploca sepium, total germination rate, germination force, vitality index, radicle growth

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