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植物研究 ›› 2006, Vol. 26 ›› Issue (4): 411-415.doi: 10.7525/j.issn.1673-5102.2006.04.015

• 论文 • 上一篇    下一篇

辣椒雄性不育系与可育系小孢子发生的细胞学观察

李莹莹1;魏佑营2*;张瑞华2;吕 杰2   

  1. (1.菏泽学院园林工程系,菏泽 274015) (2.山东农业大学园艺科学与工程学院,泰安 271018)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-07-20 发布日期:2006-07-20
  • 通讯作者: 魏佑营
  • 基金资助:
     

Cytological investigation on microsporogenesis of male sterility of pepper

LI Ying-Ying;WEI You-Ying*;ZHANG Rui-Hua;LÜJie   

  1. (1.Landscape engineering department, Heze College, Heze 274015) (2.College of Horticulture, Shandong Agricultural University, Taian 271018)
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-20 Published:2006-07-20
  • Contact: WEI You-Ying
  • Supported by:
     

摘要: 为了探讨辣椒雄性不育花药败育时期和方式,以辣椒雄性不育系1442A、13733A及其可育系为试材,进行了研究。结果发现:败育现象从造孢细胞时期以后每个阶段都有发生,败育形式有造孢细胞液泡化、畸形、拉长、细胞间隙大;绒毡层细胞径向过度伸长,高度液泡化,且出现多层细胞,严重挤压小孢子母细胞,解体较晚且充塞花粉囊室;薄壁细胞取代了药室内壁、中层、绒毡层和小孢子母细胞的分化;药室内壁、中层层数增加,绒毡层细胞肥大,造孢细胞或花粉母细胞分解解体;由于花粉母细胞胼胝质壁不降解而无法释放出四分体小孢子;染色浅、细胞质被降解成空壳的单核期小孢子因缺乏营养物质而败育。

关键词: 辣椒, 雄性不育, 小孢子发生, 细胞学

Abstract: Cytological investigation on microsporogenesis of male sterility of pepper were studied, the results were as following: Abortion was found from multiplication stage of sporogenous cells.Sporogenous cells were vacuolated, misshapen, stretched-out and the clearance between cells was enlarged. Tapetum cells were elongated, vacuolated or tapetum was made up of multilayer cells or discomposed tardily. The structure of powder chamber was abnormal and replaced by indifferentiated cells full of thin cytoplasm. Cell layers of powder chamber wall and middlelevel were increased, tapetum cells were hypertrophied and sporogenous cells or microspore mother cells were disaggregated. Quartered microspore couldn’t be released because the callose wall of microspores couldn’t decompose. Cytoplasm of monocytic microspore were decompounded.

Key words: pepper, male sterility, microsporogenesis, cytology

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