天山雪莲SikGLP基因的克隆及表达分析

doi:10.7525/j.issn.1673-5102.2013.04.013

植物研究 ›› 2013, Vol. 33 ›› Issue (4): 461-467.doi: 10.7525/j.issn.1673-5102.2013.04.013

天山雪莲SikGLP基因的克隆及表达分析

刘超¹；张林华¹；邱红林¹；王爱英¹；胡鸢雷²；祝建波^1*

1.石河子大学农业生物技术重点实验室，石河子　832003；2.北京大学生命科学学院，北京　100871

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-07-20 发布日期:2013-07-20
通讯作者: 祝建波
基金资助:

Cloning and Expression Analysis of SikGLP Gene in Sasussured involucrata Kar. et Kir

LIU Chao;ZHANG Lin-Hua;QIU Hong-Lin;WANG Ai-Ying;HU Yuan-Lei;ZHU Jian-Bo*

1.Key Laboratory of Agricultural Biotechnology of Shihezi University,Shihezi　832003；2.College of Life Sciences,Beijing University, Beijing　100871

Received:1900-01-01 Revised:1900-01-01 Online:2013-07-20 Published:2013-07-20
Contact: ZHU Jian-Bo
Supported by:

摘要/Abstract

摘要： 从天山雪莲cDNA文库中分析得到一个类萌发素蛋白基因序列，命名为SikGLP。生物信息学分析表明，该基因包含1个633 bp的完整开放阅读框，编码210个氨基酸。SikGLP分子量为21.6 kDa，理论等电点是6.81，并且是具有信号肽的疏水性蛋白。氨基酸多序列比对发现GLP序列保守性较高，具有的GLP的典型特征。17个物种间的系统发育树可以看出与葡萄GLP进化关系最近。Real time-PCR检测表明，SikGLP基因受低温、甲基紫精和PEG诱导表达，可能在胁迫初期对提高植物防御起作用。

关键词: 天山雪莲, 类萌发素蛋白, 实时定量PCR

Abstract: A novel germin-like protein gene clone was analyzed from a cDNA library of Sasussured involucrate, named SikGLP. Bioinformatics analysis showed that the SikGLP gene contains a 633 bp open reading frame encoding 210 amino acids with a theoretical molecular mass of 21.6 kDa and an isoelectric point of 6.81. SikGLP was a hydrophobic protein and contained a signal peptide. Multiple sequence alignment of GLP proteins revealed that the GLP gene shares high identities in sequence, and possesses typical characteristics of plant germin-like proteins. Phylogenetic analysis among 17 species indicated that SikGLP was clustered with Vitis vinifera. Real time-PCR analysis showed that the expression of SikGLP gene by low temperature, MV and PEG stresses. It may plays a role in improving resistance capacity of early stress.

Key words: Sasussured involucrata Kar.et Kir, germin-like proteins, real time-PCR

中图分类号:

S567.1+9

刘超;张林华;邱红林;王爱英;胡鸢雷;祝建波*. 天山雪莲SikGLP基因的克隆及表达分析[J]. 植物研究, 2013, 33(4): 461-467.

LIU Chao;ZHANG Lin-Hua;QIU Hong-Lin;WANG Ai-Ying;HU Yuan-Lei;ZHU Jian-Bo*. Cloning and Expression Analysis of SikGLP Gene in Sasussured involucrata Kar. et Kir[J]. Bulletin of Botanical Research, 2013, 33(4): 461-467.

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天山雪莲SikGLP基因的克隆及表达分析

Cloning and Expression Analysis of SikGLP Gene in Sasussured involucrata Kar. et Kir

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