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    15 December 2003, Volume 23 Issue 4
    A NEW SPECIES OF CORYDALIS (FUMARIACEAE) FROM JILIN PROVINCE
    WANG Ying-Wei, LIU Quan-Ru, ZHANG Ming-Li
    2003, 23(4):  385-387. 
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    A new species of the genus Corydalis (Fumariaceae) collected from Jilin Province is reported.
    TWO NEW VARIETIES OF ARTEMISIA OXYCEPHALA FROM NORTHEAST CHINA
    LI Ji-Yun
    2003, 23(4):  388-389. 
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    Two new varieties of Artemisia oxycephala Kitag. from Northeast China are given, i. e. Artemisia oxycephala Kitag. var. sporadantha W. Wang and A. oxycephala Kitag. var. aureinitens W. Wang.
    TWO NEW VARIETIES FROM DAXING'ANLING
    ZHANG Yue, MA Jun-Ying
    2003, 23(4):  390-390. 
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    Two varieties collected from Daxing'anling:Boschniakia rossica var. flavida Y.Zhang et J.Y.Ma. and Chamaenerion angustifolium var. albium Y.Zhang et J.Y.Ma.
    A NEW VARIETY OF THE GENUS OXYTROPIS FROM QINGHAI PROVINCE, CHINA
    WU Yu-Hu
    2003, 23(4):  391-391. 
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    A new variety of Oxytropis from Qinghai Province are reported.
    NEW RECORDS OF AMBLYSTEGIACEAE IN YUNNAN PROVINCE, CHINA
    WU Yu-Huan, GAO Chien
    2003, 23(4):  392-395. 
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    species and three varieties are reported here as new records for Yunnan province, China. Among of them, Drepanocaldus tenuinervis Kop. is new to China. The general habitats and geographical distributions of these species are also discussed.
    A SPECIES OF DESMARESTIA (DESMARESTIACEAE) NEW TO CHINA
    LUAN Ri-Xiao, CHU Zhi-Guang, SU Qiao, AN Li-Jia
    2003, 23(4):  396-398. 
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    This paper is taxonomic study of Desmarestia ligulata Lamouroux (Desmarestiaceae, Phaeophyta) from the coast Dalian.This species is new to China.
    SOME NEWLY RECORDED PLANTS FROM HUNAN PROVINCE OF CHINA (Ⅴ)
    HU Guang-Wan, LIU Ke-Ming, LIU Lin-Han
    2003, 23(4):  399-401. 
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    Some taxa, including 10 species, Epimedium brachyrrhizum Stearn, Impatiens wuyuanensis Y. L. Chen, Actinidia laevissima C. F. Liang, Tephroseris pierotii (Miq.) Holub, Allium ovalifolium Hand. Mzt., Calanthe mannii Hook. f., Calanthe argenteo striata C. Z. Tang et S. J. Cheng, Calanthe lechangensis Z. H. Tsi et T. Tang, Liparis distans C. B. Clarke, Eragrostis unioloides (Retz.) Nees ex Steud. and 1 variety, Hylomecon japonica var. dissecta (Franch. et Savat.) Fedde, are reported as new records in the Hunan Province.
    A REEVALUATION OF THE STATUS OF POTANINIA BASED ON INTERNAL TRANSCRIBED SPACER SEQUENCES
    ZHAO Yi-Zhi, CHENG Wen-Lian, YIN Jun, CAO Rui, ZHANG Jing-Qiu
    2003, 23(4):  402-406. 
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    Because of its special feature of morphology, the phylogenetic status of Potaninia is still argued today.The ITS sequences of six species of Rosoideae and a species of Prunoideae are obtained in the study. The other five ITS sequences of five species of Rosaceae are gotten from Genbank and analyzed together. The result of comparing ITS1+ITS2 sequences of 12 plants shows that the sizes of sequences of different plants are different greatly. The sequences of ITS1 range from 224~266 bp. The sequences of ITS2 range from 202~221 bp. The result of phylogenetic analysis with PAUP 3.1.1 shows that Dasiphora is the closest genus with Potaninia;the second is Chamaerhodos;the third is Sibbaldia. The mutual feature of the three genera is that style grows from the end or side of ovary. Traditionally, Potaninia is arranged near Rosa and Agrimonia judged by torus like chalis or jar, which is not consistent with our result. The phylogenetic tree indicates that Prunus mongolica and Prunus cerasifera form a monophiletic taxa obviously, which show the relationship of Potaninia mongolica and Prunus mongolica is very far. The study also indicate Potaninia mongolica, a axerophilous floristic element of Mongolian Plateau, is derived from D.glabra, a mesophytic floristic element of East Asia;All morphological characteristics and ecological habit are the result adapting itself to dry climate of desert for ages. The discovery of derivation and migrating route provide the new proof for studying the formation of desert flora and vegetation of midst of Asia.
    ABSERVATION OF DOUBLE FERTILIZATION OF PASSIFLORA EDULIS F. FLAVICARPA DEG
    ZENG Hua-Jin, QIN Yun-Xia, LIU Zhi-Xin, PENG Cun-Zhi
    2003, 23(4):  407-409. 
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    The double fertilization process of Passiflora edulis f. flavicarpa Deg. was observed via paraffin sections staining with Haematoxylin and feulgen reaction, and the results are as follows:After pollination, pollen grains germinated soon on the stigma within 30 minutes, and then the pollen tubes grew down the transmitting tissues of the styles. After 6.5 hours, the pollen tubes reached the ovary and grew along inner wall of the ovary. After 7~7.5 hours, the pollen tubes ran through the nucellar beak to the embryo sac and relesed the contents with two sperms.In the most ovules after 7.5~9 hours of pollination, the sperms were attached to the egg cell plasmas membrane, then entered into the nucleus.The sperm chromatin showed dispersion and the male nucleolus appeared.The male and female nucleoli were combined and fused to form a single nucleolus, and the egg cell came to the fertilized.Fertiliztion of polar nucleus is in the same time and similar to the egg cell fertilization.The fertilization of P. edulis f. flavicarpa Deg. is of premitosis type. Dormant period of fertilized egg cells and primary endosperm nucleus was not observed.Cell division take place immediately after fertilization finished.
    COMPARATIVE OBSERVATIONS ON MORPHOLOGICAL CHARACTERISTICS OF POLLEN OF CULTIVAR OF PEAR IN HEILONGJIANG PROVINCE
    LIU Yan-Jie, XING Yi
    2003, 23(4):  410-414. 
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    The pollen microscopy of 20 pear cultivable varieties(strains)in Heilongjiang Province, wire observed systematically with the aid of scanning electron microscope and optical microscope. Cluster analysis was carried out to study the useful value of palynology in the study of identification of variety and parent select ion.The results showed that the pollen size, shape and surface sculpture of the same variety in pollen characteristics were stable.but there were certain differences exist in different variety.The transmutation in surface sculpture of filial generation showed that they were diversity in coefficient of relation of variety.The surface sculpture observation integrated with cluster analysis was a resultful method to group varieties of pear with the aid of palynology.the great value to put in practical of production.
    KARYOTYPE STUDIES OF FOUR SPECIES OF PORELLACEAE FROM CHINA
    YANG Xiao-Jie, SHA Wei, XIAO Jing, WANG Mei-Zhi
    2003, 23(4):  415-418. 
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    THE RELATIONSHIP BETWEEN MICROTUBULES AND FORMATION OF ENDOSPERM CELL WALLS
    GAO Xin-Qi, MA Wen-Xiang
    2003, 23(4):  419-423. 
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    Several viewpoints about the formation of endosperm cell wall at different stages in endosperm development of angiosperm including formation of syncytium, primary anticlinal and periclinal walls formation, endosperm cell mitosis and the advances of relationship between microtubules and the formation of endosperm cell walls are described in this paper. And the differences in microtubular mitotic cycle between free nucleus or cell of endosperm and meristem are listed as well.
    ON MORPHOLOGY AND MORPHOGENESIS OF SILICA BODIES IN BAMBUSA MULTIPLEX VAR. NANA
    CHEN Chang-Bin, NI Bing, XU Shou-Peng, MA Qing-Hai
    2003, 23(4):  424-428. 
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    The silica body morphology and morphogenesis of Bambusa multiplex var. nana was examined by means of light microscope(LM) and scanning electronic microscope(SEM).There are varied types of silica bodies in matural plants of Bambusa multiplex var. nana. Nodular shape in leaf blade; saddle and subcuboid in sheath; dumb-bell in the tip of culm sheath; saddle in culm sheath; elliptic in culm. No regular morphological silica bodies in roots and stems under ground. All matural silica bodies are vertical to veins.The steps of silica bodies development are:morphology from amorphous, to subround, subcuboid, then nodular or dumb-bell or saddle; size from little to bigger; arrangement to vein from mixed to vertical. The silica bodies are varied in different parts of a same matural leaf, saddle shape in sheath, subcuboid in pesudopetiole and nodular shape in leaf blade respectively. Each silica body has congregated granules in its body as a nuclear.The granules in every stage of developing plant, the changes of silica bodies morphology and size show that the silica bodies are developed gradually with the plants growing. If it was provided as a scientific basis for taxonomy, archaeology, geology study, it must be paid more attention to the changes of silica bodies.
    THE ISSR-PCR REACTION SYSTEM'S ESTABLISHMENT ABOUT SEED'S ENDOSPERM OF FIVE NEEDLES PINE
    LÜ Yan-Fang, LIU Gui-Feng, JIANG Jing, HOU Ying-Jie
    2003, 23(4):  429-432. 
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    DNA was extracted from the endosperm of five needles pine by improved CTAB method. The purity of DNA that extracted by this method was high, which was tested by UV spectrophotometer. The yield of single seed's endosperm DNA almost could meet the need of mass PCR amplification. The best stable reduplicative ISSR-PCR reaction systems of five needles pine and PCR amplification parameters were established. Sixteen ISSR-PCR primers that had vivid amplification band and abundant polymorphism had been screened. The work provides a standard program for using need's endosperm of pine to carry out establishment of genetic fingerprinting and other studies of molecular biology in the future.
    CLONING OF HAL1 GENE FROM SCCHAROMYCES CEREVISIAE AS2.375 AND THE CONSTRUCTION OF PLANT EXPRESSION VECTOR OF HAL1 GENE
    FU Chang, YANG Chuan-Ping, LIU Gui-Feng, LI Shu-Juan, JIANG Jing
    2003, 23(4):  433-436. 
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    HAL1 gene is an important salt tolerant gene in yest.Total genomic DNA was isolated from Sccharomyces cerevisiae AS2.375.An about 900 bp DNA fragment was obtained with PCR technique by using the primer that designed according to the published sequence.The DNA fragment was cloned to T vector and transformed to E. coli strain JM109.The restriction map of the recombinant plasmid was analyzed and the DNA fragment was sequenced.The result showed that the entire open reading frame had been cloned and the identity of it's sequence to the published sequence is more than 99%.After cutting the HAL1 fragment from the T vector, we ligated the fragment to the pAM194 vector and obtained the plant expression vector of HAL1 gene.The transformants of tobacco with improved salt tolerant have been screened.
    EFFECT OF WATER STRESS ON ACTIVITY OF CELL DEFENSE ENZYMES AND OSMOTIC ADJUSTMENT IN EUONYMUS FORTUNEI YOUNG PLANT
    ZHAO Li-Fang, ZHANG Jin-Zheng, ZHANG Qi-Xiang, SHI Lei, LU Ren-Qiang
    2003, 23(4):  437-442. 
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    People are attaching more importance to Euonymus fortunei, which is a newly excellent climbing vine. However, the selecting experiment is staring in the face. In this study, Euonymus fortunei young plants were treated with the osmotic stress solution PEG 6000.The relation between the plant physiology indexes and plant drought tolerance were determined. The results showed that, along with time, relative water content(RWC) descended and defense enzymes activities ascended. Proline and soluble sugar content hoiked. The variety of higher drought tolerance took on higher activity of cell defense enzymes and osmotic adjustment.
    STUDY ON THE DIVERSITY OF FLAVONOIDS IN RARE-ENDANGERED ELAEAGNUS MOLLIS POPULATION
    YAN Gui-Qin, SUN Jian-Hua, WEI Kai-Yan, LI Yong-Ling, ZHANG Lian-Shui
    2003, 23(4):  443-448. 
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    The flavonoids is used molecular marker to study the relation between natural population of Elaeagnus mollis and the habitat. The flavonoid, which is important secondary metabolit that is ubiquitous in plant, was extracted by the methynol and water from the leaves of Elaeagnus mollisand was isolated by polyamide thin-layer chromatography. The band spectrum was statistical and analyzed by hierarchical cluster and PCA analysis. The reasult showed 1)that in E. molls population was stable and was as marker for studying population differentiation of E. molls. 2) Cluster analysis indicated That the flavonoids differentiation was into among 3 populations. The dissimilarity between Xiangning and Yicheng population was small by clustering in 20 value but Pinglu population in 25 value. 3) The share ratio is obviously different among populations. 4) PCA analysis indicated that flavonoids difference among population is the relationship to its environmentail factors.
    STUDY ON THE BIOECOLOGICAL CHARACTERISTICS OF PLANT COMMUNITY OF YUNCHENG SALT LAKE
    ZHANG Hai-Yan, LI Qi, SHAN Yu-Rong, ZHANG Hai-Jian
    2003, 23(4):  449-452. 
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    The structures of plant community in Yuncheng Salt Lake are very simple, and the species of it are very scarce. The community coverage varies greatly between 20%~80% and there are only one or two dominant plants within 1 m2. The types of plant communities are closely related with the salt contents of soil. Na+ contents of shoot and root in the same species decrease with the decreasing of salt contents of soil, and Na+ contents of shoot change more greatly than that in root. There are significant differences in Na+、K+ contents and Na/K ratios among different species. The ash contents of shoot also decrease with the dropping of soil salt content. Furthermore, possible mechanism of these changes are discussed.
    THE BIOLOGICAL CHARACTERS OF FLOWERING AND FRUITING OF GUANLONGDAN
    SUN Yan, WANG Chen, LIU Ming-Yuan
    2003, 23(4):  453-457. 
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    Guanlongdan is a commercial name of Chinese drug.The original plants contain 3 species of Gentiana(G. manshuricaG. scabraG. triflora).The developmental process from flower bud to fruit was observed and discussed in this paper.It was clear that the 3 specise of Gentiana are able to hybrize each other.the breeding behavior belongs to facultative selfing and the developing of the seed embryo is irregular.
    INTERSPECIFIC ASSOCIATION BETWEEN WOODY PLANTS IN SHENWEIGOU OF GUANDI MOUNTAINS, SHANXI PROVINCE
    ZHANG Jin-Tun, JIAO Rong
    2003, 23(4):  458-463. 
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    Interspecific relationship of woody plants in Shenweigou of Guandi Mountains were studied by use of χ2-test and Pearson's correlation coefficient. The associations and correlations of 8 tree species and 18 scrubs in Shenweigou area and in different communities were analyzed. The results clearly show their interspecific relationships and their differences in resource utilization and niches.
    ON INTENSIVE DISTRIBUTIVE PATTERN OF PLANTAGO VIRGINICA POPULTIONS
    GUO Shui-Liang, HUANG Hua
    2003, 23(4):  464-471. 
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    The coverages and densities of Plantago virginica populations in different inhabiting periods in the campus of Zheijiang Normal University were surveyed by the successive sampling. The deviation coefficients were calculated and the pattern scale and pattern grain were analyzed. The results showed:(1)The populations of Plantago virginica surveyed were obvious clumpy distributed; (2) All the surveyed populations were found with a patch of 30~50 cm in diameter including patch gap;(3)The number of the population patches followed an order as less→more→less, which was related to their seed spread characteristics;(4)negative correlation between the coverages of the populations and their deviation coefficients was found, which showed that the populations of Plantago virginica became less clumping distributed in appearance with the time after their inhabiting.
    THE FLORA OF MACAU
    XING Fu-Wu, QIN Xin-Sheng, YAN Yue-Hong
    2003, 23(4):  472-477. 
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    The authors summarize the natural conditions and vegetation of Macau by surveying in the field and collecting others study papers. The constitutions features and areal types of the vascular flora in Macau are studied detailedly. And the Macau flora is compared with that of other near regions. Some new recorded plants in Macau are summerrized. In the end, the authors separate the flora of Macau into 3 parts according to the features of plants distributing and physical geography.
    ANALYSIS ON THE FLORA OF SEED PLANTS IN ELAEAGNUS MOLLIS AREAL, SHANXI PROVINCE
    ZHANG Feng
    2003, 23(4):  478-484. 
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    Elaeagnus mollis is an endemic to China, being one of the China protected two grade rare and endangered plants, and distributed mainly in hills and lower mountains in southern Shanxi. E. mollis areal is located in hills of the southern tip of Luliang Ranges and the middle and western of Zhongtiao Ranges in Shanxi, 110°36'~111°56' E, 34°52'~36°05' N, which is the transit from the basin of southern Shanxi to the hills surrounding the basin.There are 905 species of seed plants, belonging to 449 genera and 95 families in E. mollis areal, among which gymnosperms have 2 families, 2 genera and 4 species, and angiosperms have 93 families, 447 genera and 901 species. The angiosperms are composed of dicotyledon and monocotyledon, having 78 families, 312 genera and 714 species, and 15 families, 135 genera and 191 species, respectively.The flora of genera in E. mollis areal has some typical characteristics of warm temperate deciduous broad leave forest. Among them, temperate distribution elements have 276 genera and accounting for 68.66% and tropic distribution elements have 106 genera and accounting for 26.38% in the total(excluding cosmopolitan), respectively, and both them make significant dominant or have some indicating meaning for the floristic elements and vegetation. Moreover, among the floristic elements of species, endemic species to China have 369 species, accounting for 41.65% in the total species, and makes evidently dominant. They are the major elements of the edificatos and dominant species of dominant vegetation types, such as Pinus tabulaeformis, Pinus armandi, Pinus bungeana, Quercus baronii, E. mollis, Rosa xanthina, Ostryopsis davidiana, Sophora viciifolia, Forsythia suspensa, Myripnois dioica etc. Finally, the strategies for the sustainable utilizing and protection of E. mollis resource.
    A STUDY ON COMPOSITION AND CHARACTERISICS OF FLORISTIC IN XIAOLONG MOUNTAINS
    MAO Xue-Wen, ZHANG Hai-Lin, KONG Hong
    2003, 23(4):  485-491. 
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    Xiaolong Mountains there were 2400 species of seed plants belonging to 150 families and 805 genera.The large familiesof plants (100 species) there were Compositae、Rosaceae、Gramincae、Papilionaceae, 50~100 of plants there were Ranuncuaceae、Liliaceae、Umbelliferae、Cyperaceae、Labiatae、Caprifoliaceae and the quality of them mostly is temperate.There were 15 distribution type of genera of seed plants in Xiaolong Mountains.It was dear from the figures that North temperate elements play an important role in the flora and vegetation with characteristics of the Temperate Zone to the flora.In addition the abundant monolypic species and oligotypic species and relic endemic to China also indicated characteritics of ancient in origin.
    PRELIMINARY STUDIES ON THE FLORA OF KUNYU MOUNTAIN IN SHANDONG PROVINCE
    ZHANG Xian-Qiang, ZHANG Zhi-Guo, ZHANG Shu-Ping, WANG Ren-Qing
    2003, 23(4):  492-499. 
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    Kunyu mountain is located in Jiaodong peninsula and is typical of warm Tempreate Zone. The species of plants are abundant. There are 1058 species which belong to 132 families and 571 genera. The flora is complex in geographical elements, having 15 types and 12 subtypes. There are 16 large and major families in this area, such as, Compositae, Gramineae, Leguminose, Rosaceae, Cyperaceae, Liliaceae, Cruciferae, Polygonaceae; The number of them is 285 genera and 612 species, they make up 51.2 per cent of the total genera and 59.94 per cent of the total species and become the basic frame of the flora composition of Kunyu mountain. The composition of Raunkeaer's life form are arranged, Ghanerophytes(34.40%)> Hemicryptophytes(27.13%)> Therophytes(19.66%)> Cryptophytes(9.64%)> Chamaephytes(9.17%). Based on the analysis of the dominant geographical elements of the flora. The paper has discussed the major characters of the flora as follows, (1) the species of plants is rich and it is obvious that temperate elements play important roles in this place, at the same time, the proportion of tropical elements is high. (2) The flora is diverse in distribution types and is complex in geographical elements, and the characters that the flora connects widely with others have advented. (3) The flora shows a certain old in origin and a certain low in endemicity, furthermore, having a good number of monotypic genus. (4) The species of rare and endangered plants are rich and assemble at the level of family to a certain degree.
    ANALYSIS ON THE FLORA OF SEED PLANTS IN MANGHE NATURE RESERVE, SHANXI
    ZHANG Yin-Bo, ZHANG Feng, ZHAO Yi-Shan, FAN Min-Xia
    2003, 23(4):  500-506. 
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    Manghe Nature Reserve, belongs to Zhongtiao Ranges, is located in southeast Shanxi province and between 112°22'11"~112°31'35"E, 35°12'30"~35°17'20"N. There is a typical warm temperate semi wetness continental monsoon climate in the area, which is hot and rainy in summer, but mid cooler in winter. It is now known that there are 866 species of seed plants in the region, which belongs to 435 genera and 103 families. Among them there are about 3 families, 5 genera, 6 species in Gymnospermae and 100 families, 430 genenra, 860 species in Angiospermae (90 families, 346 genera, 748 species in Dicotyledoneae and 10 families, 66 genera, 112 species in Monocotyledoneae).Analysis based on families, genera and species shown that the floristic elements in the region were diversity. Among which Tropic-Subtropic-Temperate (45 families, accounting for 43.69% in total families) was dominant in the families; and Temperate floristic elements (264 genera, accounting for 60.69% in total genera) made significant dominant in genera, in which North Temperate elements(136 genera, accounting for 31.26% in total genera) is the largest among the genera; moreover, the species of endemic to China (305 species, accounting for 35.22% in total species) was more than the others, such as Pinus tabulaeformis, Pinus bungeana, Populus cathayana, Betula albo-sinensis, Quercus baronii, Quercus aliena var. acuteserrata, Pteroceltis tatarinowii, Ostryopsis davidiana and Viburnum schensianum, and Temperate Asia (190 species, accounting for 21.94% in total species) and East Asia (154 species, accounting for 17.78% in total species) elements were on second and third place. Furthermore there are 8 species of rare and endangered plant of state protected in Manghe Nature Reserve, such as Taxus mairei, Cerdidiphyllum japonicum, Archineottia gaudissarti and Gastrodia elata.
    PRELIMINARY STUDY ON SPERMATOPHYTA FLORA OF HUANGFUSHAN NATURE RESERVE IN ANHUI PROVINCE
    WANG Xiao-Peng, GAO Lin, GAO Xi-Quan, XU Ru-Song, WU Chang-Ting, ZHOU Zong-Yun
    2003, 23(4):  507-512. 
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    There are 1007 species of spermatophyta being subordinate to 529 genera, 139 families in Huangfushan Nature Reserve. Among them, Gymnosperm has 6 families, 16 genera, 31 species;Angiosperm has 133 families, 513 genera, 976 species. The composition of seed plant flora and areal ypes of genera was analysed, and the genera between Huangfushan flora and neighbouring flora was compared.It is concluded that the flora of Huangfushan Nature Reserve has rich species, many endemic and rare plants and that the origin of the flora is from far ancientry, and that the floristic elements are complex and varied; The flora has a obvious characteristic of East China flora, and approach upper edge of East China flora.